PCB 9 exposure induces endothelial cell death while increasing intracellular calcium and ROS levels

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dc.authoridYilmaz, Bayram/0000-0002-2674-6535
dc.authoridSandal, Suleyman/0000-0002-8916-3329
dc.authorwosidYilmaz, Bayram/IXD-3395-2023
dc.authorwosidSandal, Suleyman/AAA-6388-2021
dc.contributor.authorYilmaz, Bayram
dc.contributor.authorSandal, Suleyman
dc.contributor.authorCarpenter, David O.
dc.date.accessioned2024-08-04T20:35:45Z
dc.date.available2024-08-04T20:35:45Z
dc.date.issued2012
dc.departmentİnönü Üniversitesien_US
dc.description.abstractCultured porcine endothelial cells were used to determine the effects of several congeners of polychlorinated biphenyls (PCBs) on cell viability and changes induced by these congeners on levels of intracellular calcium and reactive oxygen species (ROS). Cultured endothelial cells were prepared as a single suspension and run on flow cytometry. Cell viability, intracellular calcium, and ROS concentrations were simultaneously determined by using propidium iodide, Fluo-3, and dihydrorodamine, respectively. The congeners tested included two coplanar tetrachlorinated congeners that have no ortho chlorines, PCBs 77 and 80, a tri-chloro congener which does not have any ortho chlorine, PCB 39, a di-ortho, tetrachlorinated congener, PCB 52, and a mono-ortho congener, PCB 9. PCB 9 was cytotoxic at 5 mu M within 5 min of exposure, and the toxicity increased with time and concentration. None of the other congeners showed consistent cytotoxicity. The cytotoxicity was roughly correlated with elevations in cellular ROS levels, but not with changes in intracellular calcium. To the best of our knowledge, toxicity of lower chlorinated, more volatile congeners such as PCB 9 has not been previously studied. These observations may be taken to explain the elevated risk of cardiovascular disease previously reported among residents living near to hazardous waste sites containing PCBs. (c) 2011 Wiley Periodicals, Inc. Environ Toxicol, 2012.en_US
dc.description.sponsorshipInstitute for Health and the Environment; University at Albany; Scientific and Technical Research Council of Turkey (TUBITAK); NATOen_US
dc.description.sponsorshipContract grant sponsor: Institute for Health and the Environment; Contract grant sponsor: University at Albany; Contract grant sponsor: The Scientific and Technical Research Council of Turkey (TUBITAK); Contract grant sponsor: NATOen_US
dc.identifier.doi10.1002/tox.20676
dc.identifier.endpage191en_US
dc.identifier.issn1520-4081
dc.identifier.issn1522-7278
dc.identifier.issue3en_US
dc.identifier.pmid21344606en_US
dc.identifier.scopus2-s2.0-84857035528en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage185en_US
dc.identifier.urihttps://doi.org/10.1002/tox.20676
dc.identifier.urihttps://hdl.handle.net/11616/95568
dc.identifier.volume27en_US
dc.identifier.wosWOS:000300429400007en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofEnvironmental Toxicologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcytotoxicityen_US
dc.subjectcardiovascular diseaseen_US
dc.subjectinhalationen_US
dc.subjectreactive oxygen speciesen_US
dc.subjectintracellular calciumen_US
dc.titlePCB 9 exposure induces endothelial cell death while increasing intracellular calcium and ROS levelsen_US
dc.typeArticleen_US

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