Vitiligo hastalarında bağırsak permeabı·lı·tesı·nı·n serum zonulı·n düzeyı· ı·le değerlendı·rı·lmesı· ve ı·nflamatuvar sı·tokı·nler, oksı·datı·f stres belı·rteçlerı· ı·le korelasyonu
Küçük Resim Yok
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Vitiligo, deride depigmente maküller ile seyreden edinsel bir hastalıktır. Hastalığın patogenezinde otoimmünite ve oksidatif stresin önemli rol oynadığı bilinmektedir. Bazı otoimmün hastalıklarda bağırsak geçirgenliğinde artışın olduğu ve bu durumun hastalığın ortaya çıkmasında önemli bir etken olduğu kanıtlanmıştır. Bağırsak geçirgenliğini ölçen yöntemlerden biri de bağırsaktaki sıkı bağlantıların fizyolojik modülatörü olan zonulin seviyelerinin incelenmesidir. Bu çalışmamızda hasta ve sağlıklı kontrol grubunda zonulin düzeylerine bakarak vitiligo hastalığında bağırsak geçirgenliğini değerlendirmeyi amaçladık. Aynı zamanda inflamatuvar sitokinler, oksidatif stres belirteçleri ve LPS düzeyleri ile zonulinin korelasyonunu incelemeyi amaçladık. Materyal ve Metot: Çalışmamıza 41 vitiligo hastası ve 41 sağlıklı gönüllü dahil edildi. Hasta grubunun yaş, cinsiyet, hastalık süresi, ailede vitiligo öyküsü ve VASI skorları kaydedildi. Sağlıklı gönüllüler de, hasta gurubuyla yaş ve cinsiyet olarak benzer dağılımda olmasına dikkat edilerek seçildi. Hasta ve kontrol grubundan venöz kan örnekleri alınarak zonulin, LPS, IL-6, TNF-? ve oksidatif stres belirteçleri düzeyleri incelendi. Bulgular: Hasta grubun zonulin ortalaması, kontrol grubundan istatistiksel olarak anlamlı düzeyde yüksek saptandı (p<0.05). Zonulin düzeyi ile IL-6, TNF-? ve LPS düzeyleri arasında pozitif yönlü ve istatistiksel olarak anlamlı bir ilişki saptandı (p<0.05). Hasta gurubun TNF-? ve LPS düzeyleri kontrol grubuna göre anlamlı bir düzeyde yüksek saptanırken IL-6 düzeyleri açısından anlamlı bir fark yoktu. Hasta ve kontrol grubunda plazmada bakılan oksidatif stres belirteçleri düzeyi açısından anlamlı bir fark saptamadık. Hasta grubunda kontrol grubuna göre eritrositlerde incelenen GSH, NO, TAS düzeyleri düşük, MDA düzeyi yüksek saptanırken; MPO, TOS, OSI düzeyleri açısından ise anlamlı bir fark saptamadık. Sonuç: Vitiligo hastalığında, serum zonulin seviyesinin yükseldiğini ve bağırsak geçirgenliğinin arttığını düşünmekteyiz.
Aim: Vitiligo is an acquired disease with depigmented macules on the skin. It is known that autoimmunity and oxidative stress play an important role in the pathogenesis of the disease. It has been proven that there is an increase in intestinal permeability in some autoimmune diseases and this is an important factor in the emergence of the disease. One of the methods to measure intestinal permeability is to examine the levels of zonulin, a physiological modulator of tight junctions in the intestine. In this study, we aimed to evaluate intestinal permeability in vitiligo disease by looking at zonulin levels in the patient and healthy control group. At the same time, we aimed to examine the correlation of zonulin with inflammatory cytokines, oxidative stress markers and LPS levels. Material and Method: 41 vitiligo patients and 41 healthy volunteers were included in our study. Age, gender, disease duration, family history of vitiligo and VASI scores of the patient group were recorded. Healthy volunteers were selected by paying attention to the fact that they were similar in age and gender with the patient group. Venous blood samples were taken from the patient and control groups, and the levels of zonulin, LPS, IL-6, TNF-? and oxidative stress markers were examined. Results: The mean zonulin level of the patient group was found to be statistically significantly higher than the control group (p<0.05). A positive and statistically significant correlation was found between zonulin level and IL-6, TNF-? and LPS levels (p<0.05). While the TNF-? and LPS levels of the patient group were found to be significantly higher than the control group, there was no significant difference in IL-6 levels. We did not detect a significant difference in the level of oxidative stress markers in the plasma of the patient and control groups. While the GSH, NO, TAS levels in the erythrocytes were lower and MDA levels were higher in the patient group compared to the control group; We did not find any significant difference in MPO, TOS, and OSI levels. Conclusion: We think that in vitiligo disease, serum zonulin level rises and intestinal permeability increases.
Aim: Vitiligo is an acquired disease with depigmented macules on the skin. It is known that autoimmunity and oxidative stress play an important role in the pathogenesis of the disease. It has been proven that there is an increase in intestinal permeability in some autoimmune diseases and this is an important factor in the emergence of the disease. One of the methods to measure intestinal permeability is to examine the levels of zonulin, a physiological modulator of tight junctions in the intestine. In this study, we aimed to evaluate intestinal permeability in vitiligo disease by looking at zonulin levels in the patient and healthy control group. At the same time, we aimed to examine the correlation of zonulin with inflammatory cytokines, oxidative stress markers and LPS levels. Material and Method: 41 vitiligo patients and 41 healthy volunteers were included in our study. Age, gender, disease duration, family history of vitiligo and VASI scores of the patient group were recorded. Healthy volunteers were selected by paying attention to the fact that they were similar in age and gender with the patient group. Venous blood samples were taken from the patient and control groups, and the levels of zonulin, LPS, IL-6, TNF-? and oxidative stress markers were examined. Results: The mean zonulin level of the patient group was found to be statistically significantly higher than the control group (p<0.05). A positive and statistically significant correlation was found between zonulin level and IL-6, TNF-? and LPS levels (p<0.05). While the TNF-? and LPS levels of the patient group were found to be significantly higher than the control group, there was no significant difference in IL-6 levels. We did not detect a significant difference in the level of oxidative stress markers in the plasma of the patient and control groups. While the GSH, NO, TAS levels in the erythrocytes were lower and MDA levels were higher in the patient group compared to the control group; We did not find any significant difference in MPO, TOS, and OSI levels. Conclusion: We think that in vitiligo disease, serum zonulin level rises and intestinal permeability increases.
Açıklama
Anahtar Kelimeler
Dermatoloji, Dermatology
