Genotoxicity profiles in exfoliated human mammary cells recovered from lactating mothers in Istanbul; relationship with demographic and dietary factors

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dc.authoridSandal, Suleyman/0000-0002-8916-3329
dc.authoridYilmaz, Bayram/0000-0002-2674-6535;
dc.authorwosidSandal, Suleyman/AAA-6388-2021
dc.authorwosidYilmaz, Bayram/IXD-3395-2023
dc.authorwosidTuğ, Niyazi/HLH-6301-2023
dc.contributor.authorYilmaz, Bayram
dc.contributor.authorSandal, Suleyman
dc.contributor.authorAyvaci, Habibe
dc.contributor.authorTug, Niyazi
dc.contributor.authorVitrinel, Ayca
dc.date.accessioned2024-08-04T20:36:12Z
dc.date.available2024-08-04T20:36:12Z
dc.date.issued2012
dc.departmentİnönü Üniversitesien_US
dc.description.abstractWe have investigated the presence of DNA damage in human mammary epithelial cells collected from healthy lactating mothers (age, 20-35 years) who were resident in the Istanbul area. Breast milk (10 ml) was collected from 30 women between one and two weeks post-partum. Demographic information (parity, breast cancer, occupation, duration of residency in Istanbul, consumption of fish, beef and poultry) was also obtained. Milk samples were diluted 1:1 with RPMI 1640 medium and centrifuged to collect cells. The cells were re-suspended and cell viability was determined by use of 0.4% trypan blue. DNA damage was assessed by use of the comet assay (alkaline single-cell gel electrophoresis). Fifty cells per slide and two slides per sample were scored to evaluate DNA damage. The cells were visually classified into four categories on the basis of extent of migration: undamaged (UD), lightly damaged (LD), moderately damaged (MD) and highly damaged (HD). Total comet scores (TCS) were calculated as: 1x UD + 2x LD + 3x MD + 4x HD. Exfoliated mammary cells of the donors showed high (TCS >= 150 a.u.), moderate and low DNA damage in 10 (33.3%), 8 (26.7%) and 12 (40%) mothers, respectively. There was no significant correlation between TCS for DNA damage and the duration of previous breastfeeding, parity or age. None of the mothers was vegetarian, smoker or on any medication. Meat and chicken consumption did not significantly correlate with the TCS values. Fish consumption was significantly correlated with TCS results (Spearman's rho = 0.39, p < 0.05). No significant correlation was found between the DNA-damage scores and the period of residency in Istanbul, but fish consumption increased as the duration of stay was longer (Spearman's rho = 0.53, p < 0.01). These findings suggest that the primary causes of differences in genotoxicity detected in lactating mothers in Istanbul may be of dietary origin. Our experience also confirms that sampling breast milk from lactating mothers provides a valuable and non-invasive tool to study DNA damage in mammary cells. (c) 2012 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTUBITAK [104-T-240]en_US
dc.description.sponsorshipThis study is supported by TUBITAK (Project #104-T-240). The authors would like to thank Dr. Cemil Colak of Inonu University Medical School for his advice in the statistical evaluation of the data.en_US
dc.identifier.doi10.1016/j.mrgentox.2012.06.011
dc.identifier.endpage22en_US
dc.identifier.issn1383-5718
dc.identifier.issn1879-3592
dc.identifier.issue1-2en_US
dc.identifier.pmid22885593en_US
dc.identifier.scopus2-s2.0-84869114267en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage17en_US
dc.identifier.urihttps://doi.org/10.1016/j.mrgentox.2012.06.011
dc.identifier.urihttps://hdl.handle.net/11616/95854
dc.identifier.volume749en_US
dc.identifier.wosWOS:000312360100002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofMutation Research-Genetic Toxicology and Environmental Mutagenesisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDNAen_US
dc.subjectComet assayen_US
dc.subjectBreastfeedingen_US
dc.subjectGenotoxicityen_US
dc.subjectDietary factorsen_US
dc.titleGenotoxicity profiles in exfoliated human mammary cells recovered from lactating mothers in Istanbul; relationship with demographic and dietary factorsen_US
dc.typeReview Articleen_US

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