Evaluation of hypoxia pathway genes and serum parameters in new coronavirus pneumonia (COVID-19)

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dc.contributor.authorOterkus, Mesut
dc.contributor.authorPala, Mukaddes
dc.contributor.authorYilmaz, Senay Gorucu
dc.contributor.authorTanriverdi, Elif Seren
dc.contributor.authorGunduz, Ayten
dc.contributor.authorDelen, Leman Acun
dc.contributor.authorOzturk, Dilara Altay
dc.date.accessioned2026-04-04T13:35:07Z
dc.date.available2026-04-04T13:35:07Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractBackground: Coronavirus disease-2019 (COVID-19) causes severe hypoxemia. Unlike normal pneumonia, pneumonia due to COVID-19 causes oxygen deprivation without breathing difficulties (i.e., silent hypoxia). We evaluated the relationship between COVID-19 and hypoxemia and examined possible mechanisms of pneumonia from the perspective of gene expression (HIF1A, vascular endothelial growth factor [VEGF], NF-kB, MEKK1, and EGFR) using real-time PCR and ELISA for serum parameters. Methods: We evaluated 100 individuals (50 patients and 50 controls). The patients were individuals with respiratory symptoms and pneumonia who were (COVID)-D-center dot-19 positive. The relative quantification of standardized samples wa s calculated according to the formula 2-Delta Delta CT. Receiver operating curve (ROC) analysis was made to define the diagnostic power of the genes. The expression changes of four genes in the hypoxia pathway were significant (excluding VEGF) and upregulated in the patients' serums. Results: The fold change values of the HIF1A, VEGF, NF-kB, MEKK1, and EGFR genes were 0.048, 0.688, 0.168, 0.207, and 0.171, respectively, in the cases checked against to the controls. The areas under the ROC values indicating the diagnostic power of the genes were 0.727, 0.538, 0.815, 0.734, and 0.936, respectively. Some serum parameters were significant (age, PCR, urea, LDH, WBC, ferritin, and pO2). Conclusions: The upregulation of some genes in the hypoxia pathway in COVID-19 pneumonia shows that these genes and protein products are candidates for treatment targets. At the same time, the high discriminative power of two genes (NF-kappa B and EGFR) in patients compared to controls indicates their diagnostic potential in serum samples.
dc.description.sponsorshipScientific Research Projects Unit of Malatya Turgut Ozal University [:2021/29]
dc.description.sponsorshipThis work was supported by the Scientific Research Projects Unit of Malatya Turgut Ozal University (Project No:2021/29) .
dc.identifier.doi10.1016/j.gene.2025.149395
dc.identifier.issn0378-1119
dc.identifier.issn1879-0038
dc.identifier.orcid0000-0003-0523-7819
dc.identifier.orcid0000-0003-1025-7662
dc.identifier.orcid0000-0003-1126-7416
dc.identifier.orcid0000-0003-2031-9978
dc.identifier.pmid40086704
dc.identifier.scopus2-s2.0-105001697187
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.gene.2025.149395
dc.identifier.urihttps://hdl.handle.net/11616/109615
dc.identifier.volume955
dc.identifier.wosWOS:001464421100001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofGene
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250329
dc.subjectCovid-19
dc.subjectSARS-CoV-2
dc.subjectHypoxemia
dc.subjectGene expression
dc.subjectPneumonia
dc.subjectSerum biochemistry
dc.titleEvaluation of hypoxia pathway genes and serum parameters in new coronavirus pneumonia (COVID-19)
dc.typeArticle

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