Effect of Vitreoscilla hemoglobin on production of a chemotherapeutic enzyme, L-asparaginase, by Pseudomonas aeruginosa

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dc.authorscopusid6602871282
dc.authorscopusid6602952964
dc.authorscopusid6507504123
dc.authorscopusid58448797900
dc.authorscopusid6507694541
dc.authorscopusid7004914053
dc.contributor.authorGeckil H.
dc.contributor.authorGencer S.
dc.contributor.authorAtes B.
dc.contributor.authorOzer U.
dc.contributor.authorUckun M.
dc.contributor.authorYilmaz I.
dc.date.accessioned2024-08-04T20:02:36Z
dc.date.available2024-08-04T20:02:36Z
dc.date.issued2006
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe production of L-asparaginase, an enzyme widely used in cancer chemotherapy, is mainly regulated by carbon catabolite repression and oxygen. This study was carried out to understand how different carbon sources and Vitreoscilla hemoglobin (VHb) affect the production of this enzyme in Pseudomonas aeruginosa and its VHb-expressing recombinant strain (PaJC). Both strains grown with various carbon sources showed a distinct profile of the enzyme activity. Compared to no carbohydrate supplemented medium, glucose caused a slight repression of L-asparaginase in P. aeruginosa, while it stimulated it in the PaJC strain. Glucose, regarded as one of the inhibitory sugars for the production L-asparaginase by other bacteria, was determined to be the favorite carbon source compared to lactose, glycerol and mannitol. Furthermore, contrary to common knowledge of oxygen repression of L-asparaginase in other bacteria, oxygen uptake provided by VHb was determined to even stimulate the L-asparaginase synthesis by P. aeruginosa. This study, for the first time, shows that in P. aeruginosa utilizing a recombinant oxygen uptake system, VHb, L-asparaginase synthesis is stimulated by glucose and other carbohydrate sources compared to the host strain. It is concluded that carbon catabolite and oxygen repression of L-asparaginase in fermentative bacteria is not the case for a respiratory non-fermentative bacterium like P. aeruginosa. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.en_US
dc.identifier.doi10.1002/biot.200500048
dc.identifier.endpage208en_US
dc.identifier.issn1860-6768
dc.identifier.issue2en_US
dc.identifier.pmid16892249en_US
dc.identifier.scopus2-s2.0-33750599693en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage203en_US
dc.identifier.urihttps://doi.org/10.1002/biot.200500048
dc.identifier.urihttps://hdl.handle.net/11616/91822
dc.identifier.volume1en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.relation.ispartofBiotechnology Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBacterial hemoglobinen_US
dc.subjectChemotherapeutic enzymesen_US
dc.subjectL-asparaginaseen_US
dc.subjectOxygen uptakeen_US
dc.subjectVitreoscilla hemoglobinen_US
dc.titleEffect of Vitreoscilla hemoglobin on production of a chemotherapeutic enzyme, L-asparaginase, by Pseudomonas aeruginosaen_US
dc.typeArticleen_US

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