The effectiveness of chitosan-mediated silencing of PDGF-B and PDGFR-? in the mesangial proliferative glomerulonephritis therapy

dc.authoridAlan, Saadet/0000-0003-2329-151X
dc.authoridŞALVA, EMINE/0000-0002-1159-5850
dc.authoridSalva, Emine/0000-0002-1159-5850
dc.authorwosidAlan, Saadet/ABH-4282-2020
dc.authorwosid/AAD-1704-2020
dc.authorwosidŞALVA, EMINE/CAH-3062-2022
dc.authorwosidSalva, Emine/ABI-2766-2020
dc.contributor.authorAlan, Saadet
dc.contributor.authorSalva, Emine
dc.contributor.authorYilmaz, Ismet
dc.contributor.authorTuran, Suna Ozbas
dc.contributor.authorAkbuga, Julide
dc.date.accessioned2024-08-04T20:46:03Z
dc.date.available2024-08-04T20:46:03Z
dc.date.issued2019
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPlatelet-derived growth factor-B (PDGF-B) is a growth factor that plays an important role in the progression of mesangial proliferative glomerulonephritis (MsPGN). PDGF-B may contribute to mesangioproliferative changes and is overexpressed in MsPGN. Recently, small interfering RNAs (siRNAs) have been widely used for gene silencing effects in experimental models of renal diseases. Nanoparticle-based therapeutics are preferred for reasons such as increasing therapeutic efficacy and reducing toxic effects caused by high doses. The distribution of nanoparticles to the kidney is a significant advantage in siRNA delivery. The aim of this study was to investigate the efficacy of chitosan/siRNA nanoplexes in silencing of PDGF-B and PDGFR-beta genes in kidney and to decrease mesangial cell proliferation and matrix accumulation in MsPGN model induced by anti-Thy-1.1 antibody. The therapeutic effects of chitosan/siPDGF-B + siPDGFR-beta nanoplexes in glomerulonephritic rats were studied by molecular, biochemical, and histopathologic evaluations. Chitosan/siPDGF-B + siPDGFR-beta nanoplexes markedly reduced PDGF-B and PDGFR-beta mRNA and protein expressions in experimental MsPGN model. Histopathologic examination results showed that the silencing of PDGF-B and its receptor PDGFR-beta led to reduction in mesangial cell proliferation and matrix accumulation. The use of chitosan/siPDGF-B + siPDGFR-beta nanoplexes for silencing the PDGF-B pathway in MsPGN can be considered as a new effective therapeutic strategy.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [1135202]en_US
dc.description.sponsorshipThis study was funded by the Scientific and Technological Research Council of Turkey (TUBITAK, 1135202).en_US
dc.identifier.doi10.1016/j.yexmp.2019.104280
dc.identifier.issn0014-4800
dc.identifier.issn1096-0945
dc.identifier.pmid31265815en_US
dc.identifier.scopus2-s2.0-85068970436en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.yexmp.2019.104280
dc.identifier.urihttps://hdl.handle.net/11616/98872
dc.identifier.volume110en_US
dc.identifier.wosWOS:000488144100015en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofExperimental and Molecular Pathologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMsPGNen_US
dc.subjectPDGF-Ben_US
dc.subjectPDGFR-betaen_US
dc.subjectsiRNAen_US
dc.subjectChitosanen_US
dc.titleThe effectiveness of chitosan-mediated silencing of PDGF-B and PDGFR-? in the mesangial proliferative glomerulonephritis therapyen_US
dc.typeArticleen_US

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