The effectiveness of chitosan-mediated silencing of PDGF-B and PDGFR-? in the mesangial proliferative glomerulonephritis therapy
dc.authorid | Alan, Saadet/0000-0003-2329-151X | |
dc.authorid | ŞALVA, EMINE/0000-0002-1159-5850 | |
dc.authorid | Salva, Emine/0000-0002-1159-5850 | |
dc.authorwosid | Alan, Saadet/ABH-4282-2020 | |
dc.authorwosid | /AAD-1704-2020 | |
dc.authorwosid | ŞALVA, EMINE/CAH-3062-2022 | |
dc.authorwosid | Salva, Emine/ABI-2766-2020 | |
dc.contributor.author | Alan, Saadet | |
dc.contributor.author | Salva, Emine | |
dc.contributor.author | Yilmaz, Ismet | |
dc.contributor.author | Turan, Suna Ozbas | |
dc.contributor.author | Akbuga, Julide | |
dc.date.accessioned | 2024-08-04T20:46:03Z | |
dc.date.available | 2024-08-04T20:46:03Z | |
dc.date.issued | 2019 | |
dc.department | İnönü Üniversitesi | en_US |
dc.description.abstract | Platelet-derived growth factor-B (PDGF-B) is a growth factor that plays an important role in the progression of mesangial proliferative glomerulonephritis (MsPGN). PDGF-B may contribute to mesangioproliferative changes and is overexpressed in MsPGN. Recently, small interfering RNAs (siRNAs) have been widely used for gene silencing effects in experimental models of renal diseases. Nanoparticle-based therapeutics are preferred for reasons such as increasing therapeutic efficacy and reducing toxic effects caused by high doses. The distribution of nanoparticles to the kidney is a significant advantage in siRNA delivery. The aim of this study was to investigate the efficacy of chitosan/siRNA nanoplexes in silencing of PDGF-B and PDGFR-beta genes in kidney and to decrease mesangial cell proliferation and matrix accumulation in MsPGN model induced by anti-Thy-1.1 antibody. The therapeutic effects of chitosan/siPDGF-B + siPDGFR-beta nanoplexes in glomerulonephritic rats were studied by molecular, biochemical, and histopathologic evaluations. Chitosan/siPDGF-B + siPDGFR-beta nanoplexes markedly reduced PDGF-B and PDGFR-beta mRNA and protein expressions in experimental MsPGN model. Histopathologic examination results showed that the silencing of PDGF-B and its receptor PDGFR-beta led to reduction in mesangial cell proliferation and matrix accumulation. The use of chitosan/siPDGF-B + siPDGFR-beta nanoplexes for silencing the PDGF-B pathway in MsPGN can be considered as a new effective therapeutic strategy. | en_US |
dc.description.sponsorship | Scientific and Technological Research Council of Turkey (TUBITAK) [1135202] | en_US |
dc.description.sponsorship | This study was funded by the Scientific and Technological Research Council of Turkey (TUBITAK, 1135202). | en_US |
dc.identifier.doi | 10.1016/j.yexmp.2019.104280 | |
dc.identifier.issn | 0014-4800 | |
dc.identifier.issn | 1096-0945 | |
dc.identifier.pmid | 31265815 | en_US |
dc.identifier.scopus | 2-s2.0-85068970436 | en_US |
dc.identifier.scopusquality | Q1 | en_US |
dc.identifier.uri | https://doi.org/10.1016/j.yexmp.2019.104280 | |
dc.identifier.uri | https://hdl.handle.net/11616/98872 | |
dc.identifier.volume | 110 | en_US |
dc.identifier.wos | WOS:000488144100015 | en_US |
dc.identifier.wosquality | Q2 | en_US |
dc.indekslendigikaynak | Web of Science | en_US |
dc.indekslendigikaynak | Scopus | en_US |
dc.indekslendigikaynak | PubMed | en_US |
dc.language.iso | en | en_US |
dc.publisher | Academic Press Inc Elsevier Science | en_US |
dc.relation.ispartof | Experimental and Molecular Pathology | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | MsPGN | en_US |
dc.subject | PDGF-B | en_US |
dc.subject | PDGFR-beta | en_US |
dc.subject | siRNA | en_US |
dc.subject | Chitosan | en_US |
dc.title | The effectiveness of chitosan-mediated silencing of PDGF-B and PDGFR-? in the mesangial proliferative glomerulonephritis therapy | en_US |
dc.type | Article | en_US |