Free radical-induced damage to DNA: Mechanisms and measurement

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dc.authoridJafar, Nadhir Najim/0000-0002-5947-8303
dc.authoridJafar, Nadhir N. A./0000-0003-2279-8781
dc.authoridJaruga, Pawel/0000-0001-9192-6084
dc.authorwosidJaruga, Pawel/M-4378-2015
dc.authorwosidJafar, Nadhir Najim/AAY-6776-2021
dc.authorwosidJafar, Nadhir N. A./N-2655-2019
dc.contributor.authorDizdaroglu, M
dc.contributor.authorJaruga, P
dc.contributor.authorBirincioglu, M
dc.contributor.authorRodriguez, H
dc.date.accessioned2024-08-04T20:13:09Z
dc.date.available2024-08-04T20:13:09Z
dc.date.issued2002
dc.departmentİnönü Üniversitesien_US
dc.description.abstractFree radicals are produced in cells by cellular metabolism and by exogenous agents. These species react with biomolecules in cells, including DNA. The resulting damage to DNA, which is also called oxidative damage to DNA, is implicated in mutagenesis, carcinogenesis, and aging. Mechanisms of damage involve abstractions and addition reactions by free radicals leading to carbon-centered sugar radicals and OH- or H-adduct radicals of heterocyclic bases. Further reactions of these radicals yield numerous products, Various analytical techniques exist for the measurement of oxidative damage to DNA. Techniques that employ gas chromatography (GC) or liquid chromatography (LC) with mass spectrometry (MS) simultaneously measure numerous products, and provide positive identification and accurate quantification. The measurement of multiple products avoids misleading conclusions that might be drawn from the measurement of a single product, because product levels vary depending on reaction conditions and the redox status of cells. In the past, GUMS was used for the measurement of modified sugar and bases, and DNA-protein cross-links. Recently, methodologies using LC/tandem MS (LC/MS/MS) and LC/MS techniques were introduced fur the measurement of modified nucleosides. Artifacts might occur with the use of any of the measurement techniques. The use of proper experimental conditions might avoid artifactual formation of products in DNA. This article reviews mechanistic aspects of oxidative damage to DNA and recent developments in the measurement of this type of damage using chromatographic and mass spectrometric techniques. Published by Elsevier Science, Inc.en_US
dc.identifier.doi10.1016/S0891-5849(02)00826-2
dc.identifier.endpage1115en_US
dc.identifier.issn0891-5849
dc.identifier.issn1873-4596
dc.identifier.issue11en_US
dc.identifier.pmid12031895en_US
dc.identifier.scopus2-s2.0-0036591855en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1102en_US
dc.identifier.urihttps://doi.org/10.1016/S0891-5849(02)00826-2
dc.identifier.urihttps://hdl.handle.net/11616/93422
dc.identifier.volume32en_US
dc.identifier.wosWOS:000175822600008en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofFree Radical Biology and Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDNA repairen_US
dc.subjecthydroxyl radicalen_US
dc.subjectmass spectrometryen_US
dc.subjectoxidative damage to DNAen_US
dc.subjectfree radicalsen_US
dc.titleFree radical-induced damage to DNA: Mechanisms and measurementen_US
dc.typeReview Articleen_US

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