Development of a HPLC Method for the Determination of Cyclosporine A From Chitosan Nanoparticles

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dc.authoridKAYNAK, Mustafa Sinan/0000-0003-2917-2407
dc.authoridBuyuktuncel, Ebru/0000-0001-7269-6594
dc.authorwosidAktaş, Yeşim/ABB-3264-2020
dc.authorwosidBuyuktuncel, Saliha Ebru/ABH-9343-2020
dc.authorwosidKAYNAK, Mustafa Sinan/D-9453-2019
dc.authorwosidYücel, Çiğdem/AAT-6484-2021
dc.contributor.authorBuyuktuncel, Ebru
dc.contributor.authorYucel, Cigdem
dc.contributor.authorKaynak, M. Sinan
dc.contributor.authorAktas, Yesim
dc.date.accessioned2024-08-04T20:36:12Z
dc.date.available2024-08-04T20:36:12Z
dc.date.issued2012
dc.departmentİnönü Üniversitesien_US
dc.description.abstractA reversed-phase high performance liquid chromatographic method was developed for the determination of cyclosporine A from chitosan nanoparticles. Ibuprofen was used as internal standard. The separation was achieved on a Phenomenex C18 column (150 x 4.6mm, 5 mu m) using 60: 20: 20, acetonitril: methanol: water mixture at pH 4 as mobile phase under isocratic conditions. The system was operated at 80 degrees C and the flow rate of mobile phase was adjusted to 1 mL/min. The detection wavelength was set at 205 nm. The calibration curve was linear from 2 to 150 mu g/mL with correlation coefficient values from 0.9994 to 0.9997. The lower limit of quantification 2 mu g/mL and limit of detection was 0.5 mu g/mL. Recovery was 99-101 % and the intra-and inter-day coefficients of variation were 0.94-2.32 and 2.52-3.45 %, respectively depending on the concentration. The method was found to be specific, accurate, precise and sensitive for the determination of cyclosporine A from chitosan based drug delivery systems.en_US
dc.description.sponsorshipErciyes University Research Council [TSA-11-3472]en_US
dc.description.sponsorshipThis research was supported by the Erciyes University Research Council (Project no: TSA-11-3472).en_US
dc.identifier.endpage1088en_US
dc.identifier.issn0326-2383
dc.identifier.issue8en_US
dc.identifier.scopus2-s2.0-84869169339en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage1083en_US
dc.identifier.urihttps://hdl.handle.net/11616/95855
dc.identifier.volume31en_US
dc.identifier.wosWOS:000208861000001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherColegio Farmaceuticos Provincia De Buenos Airesen_US
dc.relation.ispartofLatin American Journal of Pharmacyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectChitosan nanoparticlesen_US
dc.subjectCyclosporine Aen_US
dc.subjectHPLCen_US
dc.titleDevelopment of a HPLC Method for the Determination of Cyclosporine A From Chitosan Nanoparticlesen_US
dc.typeArticleen_US

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