High Genetic Diversity among Stenotrophomonas maltophilia Isolates from Single Hospital: Nosocomial Outbreaks or Genotypic Profile Changes during Subcultures

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dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authoridSüer, Kaya/0000-0002-2565-3425;
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.authorwosidSüer, Kaya/GNM-8453-2022
dc.authorwosidGÜVENİR, MERYEM/G-9669-2018
dc.contributor.authorGuvenir, Meryem
dc.contributor.authorOtlu, Baris
dc.contributor.authorTunc, Emine
dc.contributor.authorAktas, Elif
dc.contributor.authorSuer, Kaya
dc.date.accessioned2024-08-04T20:44:27Z
dc.date.available2024-08-04T20:44:27Z
dc.date.issued2018
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBackground: Stenotrophomonas maltophilia is a non-fermentative gram-negative bacillus which is widely recognised as an important nosocomial pathogen causing pneumonia, blood-stream, wound and urinary tract infections, particularly in immunosuppressed patients. The aim of this study was to evaluate a nosocomial outbreak of by S. maltophilia in an intensive care unit of a tertiary hospital and evaluate unexpected multiclonality. Methods: A total of 11 isolates from respiratory cultures in intensive care unit of a 24 bed tertiary hospital obtained over a one months period and one isolate obtained from the nebuliser during environmental screening were investigated. The bacteria were identified by Phoenix 100 system. The clonal relatedness was evaluated by PFGE and semi-automated repetitive sequence-based PCR. Genotyping tests were repeated for 10 serial subcultures. Results: PFGE and DiversiLab yielded 10 genotypic profiles for 12 isolates. Four to eight different genotypes were observed from 10 subcultures of the same isolate. Conclusion: We conclude that, high genetic diversity and supposed multiclonal appearance of the outbreak isolates may be due to changing profiles during subcultures most probably depending on hypermutation.en_US
dc.identifier.doi10.21315/mjms2018.25.2.5
dc.identifier.endpage49en_US
dc.identifier.issn1394-195X
dc.identifier.issn2180-4303
dc.identifier.issue2en_US
dc.identifier.pmid30918454en_US
dc.identifier.scopus2-s2.0-85046126664en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage40en_US
dc.identifier.urihttps://doi.org/10.21315/mjms2018.25.2.5
dc.identifier.urihttps://hdl.handle.net/11616/98257
dc.identifier.volume25en_US
dc.identifier.wosWOS:000432225300005en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherUniv Sains Malaysia, Sch Medical Sciencesen_US
dc.relation.ispartofMalaysian Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectStenotrophomonas maltophiliaen_US
dc.subjectmulticlonal outbreaksen_US
dc.subjecthypermutationen_US
dc.titleHigh Genetic Diversity among Stenotrophomonas maltophilia Isolates from Single Hospital: Nosocomial Outbreaks or Genotypic Profile Changes during Subculturesen_US
dc.typeArticleen_US

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