Quercetin dose affects the fate of hepatic ischemia and reperfusion injury in rats: An experimental research
Yükleniyor...
Dosyalar
Tarih
2018
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevıer scıence bv, po box 211, 1000 ae amsterdam, netherlands
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
BACKGROUND: Quercetin found in fruits and vegetables has an antioxidative effect. We aimed to investigate the protective effects of quercetin according to different doses on hepatic and ischemia-reperfusion (I/R) injury.
METHODS: Fifty mature male Sprague-Dawley rats were randomly divided into five groups (n = 10 for each). All the animal groups underwent laparotomy. Group 1 rats served as a sham-operated group. Groups 2-5 underwent 1 h hepatic ischemia and were followed by 2 h reperfusion. Group 3-5 animals received an additional intraperitoneal dose of 25, 50 or 100 mg/kg quercetin respectively before I/R operation. Blood samples were collected for determining serum aspartate transaminase (AST), alanine transaminase (ALT) and malondialdehyde (MDA) levels. Also, liver tissue samples were taken for measuring of liver MDA concentration and for histopathology assessment.
RESULTS: The highest levels of biochemical parameters were observed in group 2. In quercetin-treated groups, serum AST, ALT, MDA levels, and tissue MDA concentration were decreased as inversely with increasing quercetin dose. Microscopic evaluation revealed that most conspicuous histological improvement was observed in 50 mg/kg quercetin co-treated rats. 25 and 100 mg/kg quercetin co-treatment could not protect completely against hepatic I/R injury.
CONCLUSION: Quercetin can be effective in preventing of hepatic I/R injury when the correct dose was used.
Açıklama
Anahtar Kelimeler
Renal Ischemıa/Reperfusıon Injury, Induced Oxıdatıve Stress, Reactıve Oxygen, Flavonoıd Quercetın, Lıver, Pretreatment, Hepatocytes, Mechanısms, Preventıon, Protectıon
Kaynak
Internatıonal journal of surgery
WoS Q Değeri
Scopus Q Değeri
Cilt
53
Sayı
Künye
Uylas, MU. (2018). Quercetin dose affects the fate of hepatic ischemia and reperfusion injury in rats: An experimental research. Cilt:53, 117-121 ss.
