The role of neopterin in cross-talk between tumor and tumor microenvironment in hepatocellular carcinoma

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dc.contributor.authorSatilmis, Basri
dc.contributor.authorCicek, Egemen
dc.contributor.authorKarakas, Serdar
dc.contributor.authorKutluturk, Koray
dc.contributor.authorOtan, Emrah
dc.contributor.authorYilmaz, Sezai
dc.contributor.authorSahin, Tevfik Tolga
dc.date.accessioned2026-04-04T13:32:59Z
dc.date.available2026-04-04T13:32:59Z
dc.date.issued2024
dc.departmentİnönü Üniversitesi
dc.description.abstractNeopterin is a marker of activated immune response, but its role in hepatocarcinogenesis is unknown. The present study aims to evaluate the effects of neopterin on prooncogenic/proinflammatory, apoptotic pathways, and other molecular mechanisms in HCC. We used SNU449, Huh-7, SK-Hep-1, and HepG2 cell lines. A cell viability assay was performed with different concentrations of neopterin. RT-PCR, Western blotting, transwell migration, scratch assay, and reactive oxygen species (ROS) production assays were performed at inhibition concentration 50 of neopterin, which was 40 mu M for SNU449 and 80 mu M for other cell lines. There were significant changes in mTOR, STAT3, PI3K, and interleukin-6 gene expressions, which were also supported by the protein expressions. Neopterin did not affect apoptosis in SNU449, while apoptosis increased by all doses of neopterin in SK-Hep-1 and HepG2. ROS production was increased in all cell lines in response to neopterin. Cell migration was reduced in SK-Hep1 and HepG2 but did not change in SNU449 and Huh-7. Our study showed that neopterin is not just a byproduct. The results suggest that neopterin may be a paracrine factor that modulates pro-inflammatory and pro-oncogenic pathways responsible for the biological behavior of HCC in a chronic inflammatory tumor microenvironment.
dc.description.sponsorshipIdot;nonu University Scientific Research Projects Unit [TSG-2019-1686]
dc.description.sponsorshipThe present study was supported by the & Idot;nonu University Scientific Research Projects Unit (TSG-2019-1686).
dc.identifier.doi10.1515/pteridines-2022-0050
dc.identifier.issn0933-4807
dc.identifier.issn2195-4720
dc.identifier.issue1
dc.identifier.orcid0000-0002-9132-6115
dc.identifier.orcid0000-0002-2538-5774
dc.identifier.orcid0000-0003-2691-7418
dc.identifier.orcid0000-0002-7030-4953
dc.identifier.orcid0000-0002-8044-0297
dc.identifier.scopus2-s2.0-85209666754
dc.identifier.scopusqualityQ4
dc.identifier.urihttps://doi.org/10.1515/pteridines-2022-0050
dc.identifier.urihttps://hdl.handle.net/11616/108856
dc.identifier.volume35
dc.identifier.wosWOS:001350436900001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherDe Gruyter Poland Sp Z O O
dc.relation.ispartofPteridines
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjecthepatocellular carcinoma
dc.subjecttumor microenvironment
dc.subjectneopterin
dc.titleThe role of neopterin in cross-talk between tumor and tumor microenvironment in hepatocellular carcinoma
dc.typeArticle

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