Cloning and expression of the Vitreoscilla hemoglobin gene in Enterobacter aerogenes

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dc.authoridGeckil, Hikmet/0000-0003-0070-0691
dc.authorwosidStark, Benjamin C/E-6095-2010
dc.authorwosidGeckil, Hikmet/F-7647-2012
dc.contributor.authorErenler, SO
dc.contributor.authorGencer, S
dc.contributor.authorGeckil, H
dc.contributor.authorStark, BC
dc.contributor.authorWebster, DA
dc.date.accessioned2024-08-04T20:15:16Z
dc.date.available2024-08-04T20:15:16Z
dc.date.issued2004
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe hemoglobins found in unicellular organisms show a great deal of chemical reactivity, protecting cells against oxidative stress, and hence have been implicated in a wider variety of potential functions than those traditionally associated with animal and plant hemoglobins. There are well-documented studies showing that bacteria expressing Vitreoscilla hemoglobin (VHb), the first prokaryotic hemoglobin characterized, have better growth and oxygen uptake rates than their VHb counterparts. Here, the expression of VHb, its effect on the growth and antioxidant enzyme status of cells under different culture conditions was studied by cloning the complete regulatory and coding sequences (vgb) for VHb in Enterobacter aerogenes. Contrary to what has been reported for Escherichia coli, the expression of vgb in E.aerogenes decreased several fold under 10% of atmospheric oxygen (approximate to2% oxygen) and its growth was not greatly improved by the presence of VHb. Measured either as viable cells or total cell mass, untransformed E. aerogenes grew better than the recombinant strains. At the late exponential phase, however, the vgb-bearing strain was determined to have a higher cell number and total cell mass than the strain bearing only the plasmid vector with no vgb insert. The VHb expressing strain also had an oxygen uptake rate several fold higher than its counterparts. Given that oxidative stress may occur upon elevated oxygen exposure and be balanced by the action of antioxidative compounds, the level of antioxidative response of E. aerogenes expressing VHb was also studied. The VHb expressing strain had substantially (1.5-2.6-fold) higher catalase activity than strains not expressing VHb. Both VHb+ and VHb- strains, however, showed similar levels of superoxide dismutase activity. The activity of both enzymes was also growth phase dependent. Stationary phase cells of all strains showed 2-5-fold higher activity for these enzymes than cells at the exponential phase.en_US
dc.identifier.doi10.1023/B:ABIM.0000025945.53332.74
dc.identifier.endpage248en_US
dc.identifier.issn0003-6838
dc.identifier.issn1608-3024
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-3342931340en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage241en_US
dc.identifier.urihttps://doi.org/10.1023/B:ABIM.0000025945.53332.74
dc.identifier.urihttps://hdl.handle.net/11616/94284
dc.identifier.volume40en_US
dc.identifier.wosWOS:000221647800005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPleiades Publishing Incen_US
dc.relation.ispartofApplied Biochemistry and Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEscherichia-Colien_US
dc.subjectIntracellular Expressionen_US
dc.subjectBacterial Hemoglobinen_US
dc.subjectSuperoxide-Dismutaseen_US
dc.subjectOxidative Stressen_US
dc.subjectAlpha-Amylaseen_US
dc.subjectGlobin Geneen_US
dc.subjectCatalaseen_US
dc.subjectEnhancementen_US
dc.subjectDegradationen_US
dc.titleCloning and expression of the Vitreoscilla hemoglobin gene in Enterobacter aerogenesen_US
dc.typeArticleen_US

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