Genomic DNA of Nostoc commune (Cyanobacteria) becomes covalently modified during long-term (decades) desiccation but is protected from oxidative damage and degradation

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dc.authoridHelm, Richard/0000-0001-5317-0925
dc.authoridJaruga, Pawel/0000-0001-9192-6084
dc.authorwosidJaruga, Pawel/M-4378-2015
dc.authorwosidWright, Daniel/JCO-8008-2023
dc.authorwosidHelm, Richard/N-6249-2018
dc.contributor.authorShirkey, B
dc.contributor.authorMcMaster, NJ
dc.contributor.authorSmith, SC
dc.contributor.authorWright, DJ
dc.contributor.authorRodriguez, H
dc.contributor.authorJaruga, P
dc.contributor.authorBirincioglu, M
dc.date.accessioned2024-08-04T20:13:19Z
dc.date.available2024-08-04T20:13:19Z
dc.date.issued2003
dc.departmentİnönü Üniversitesien_US
dc.description.abstractGenomic DNA of Nostoc commune ( Cyanobacteria) became covalently modified during decades of desiccation. Amplification of gene loci from desiccated cells required pretreatment of DNA with N-phenacylthiazolium bromide, a reagent that cleaves DNA- and protein-linked advanced glycosylation end-products. DNA from 13 year desiccated cells did not show any higher levels of the commonly studied oxidatively modified DNA damage biomarkers 8-hydroxyguanine, 8-hydroxyadenine and 5-hydroxyuracil, compared to commercially available calf thymus DNA. Different patterns of amplification products were obtained with DNA from desiccated/ rehydrating cells and a liquid culture derived from the dried material, using the same set of primers. In contrast, a reproducible fingerprint was obtained, irrespective of time of rehydration of the DNA, using a primer (5'-GWCWATCGCC-3') based upon a highly iterated palindromic repeat sequence present in the genome. In vitro, the desiccation of cccDNA led to loss of supercoiling, aggregation, loss of resolution during agarose gel electrophoresis and loss of transformation and transfection efficiency. These changes were minimized when DNA was desiccated and stored in the presence of trehalose, a non-reducing disaccharide present in Nostoc colonies. The response of the N. commune genome to desiccation is different from the response of the genomes of cyanobacteria and Deinococcus radiodurans to ionizing radiation.en_US
dc.identifier.doi10.1093/nar/gkg404
dc.identifier.endpage3005en_US
dc.identifier.issn0305-1048
dc.identifier.issn1362-4962
dc.identifier.issue12en_US
dc.identifier.pmid12799425en_US
dc.identifier.scopus2-s2.0-0037901860en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage2995en_US
dc.identifier.urihttps://doi.org/10.1093/nar/gkg404
dc.identifier.urihttps://hdl.handle.net/11616/93540
dc.identifier.volume31en_US
dc.identifier.wosWOS:000183832000003en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherOxford Univ Pressen_US
dc.relation.ispartofNucleic Acids Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectRadical-Induced Damageen_US
dc.subjectChemical Determinationen_US
dc.subjectExcision-Repairen_US
dc.subjectMessenger-Rnaen_US
dc.subjectToleranceen_US
dc.subjectProteinen_US
dc.subjectPolysaccharideen_US
dc.subjectMechanismsen_US
dc.subjectResistanten_US
dc.titleGenomic DNA of Nostoc commune (Cyanobacteria) becomes covalently modified during long-term (decades) desiccation but is protected from oxidative damage and degradationen_US
dc.typeArticleen_US

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