Intracerebroventricular asprosin administration strongly stimulates hypothalamic-pituitary-testicular axis in rats

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dc.authoridTekin, Suat/0000-0002-2757-1802
dc.authoridKeskin, Tuba/0000-0002-9212-4021
dc.authoridKeskin, Tuba/0000-0002-9212-4021
dc.authorwosidTekin, Suat/KEI-2266-2024
dc.authorwosidKeskin, Tuba/ABC-1496-2021
dc.authorwosidKeskin, Tuba/AGX-8609-2022
dc.contributor.authorKeskin, Tuba
dc.contributor.authorErden, Yavuz
dc.contributor.authorTekin, Suat
dc.date.accessioned2024-08-04T20:50:38Z
dc.date.available2024-08-04T20:50:38Z
dc.date.issued2021
dc.departmentİnönü Üniversitesien_US
dc.description.abstractAsprosin, a protein-based secretary product of white adipose tissue, stimulates appetite hepatic glucose production. It crosses blood-brain barrier and stimulates appetite center and causes sperm chemotaxis but exact role of this endogenous agent is not completely known. This study was conducted to investigate possible effects of central asprosin infusion on the hormones involved in the hypothalamic-pituitary-testicular (HPT) axis and sperm cells. Spraque Dawley male rats were divided into four groups; control, sham, low asprosin (34) and high asprosin (68 nM) groups, (n = 10 for each group). Control group remain intact while a brain infusion kit was placed in the lateral ventricles of the rats in the sham group (artificial cerebrospinal fluid) and asprosin (34 and 68 nM) was infused for 14 days. At the end of the experiment, the hypothalamus, blood, and epididymis tissues of the rats were collected. Gonadotropin-releasing hormone (GnRH) mRNA and tissue protein levels were determined in the hypothalamus tissue by RT-PCR and Western Blot methods. Serum luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone levels were examined using the ELISA method from blood samples and sperm cells were examined in the epididymis tissue. GnRH mRNA and protein expressions of asprosin administered groups were higher than control and sham groups (p < 0.05). Asprosin infusion was also found to increase serum FSH, LH, and testosterone levels (p < 0.05). In addition, sperm density, motility, and progressive movement were observed to increase in asprosin administered groups (p < 0.05). This study suggests that central asprosin stimulate the HPT axis and also epididymis tissue. Our results implicates potential role for asprosin in male infertility.en_US
dc.description.sponsorshipScientific Research Projects unit of Inonu University [TYL-2020-2185]en_US
dc.description.sponsorshipThis study was supported by the Scientific Research Projects unit of Inonu University (Project number: TYL-2020-2185) .en_US
dc.identifier.doi10.1016/j.mce.2021.111451
dc.identifier.issn0303-7207
dc.identifier.issn1872-8057
dc.identifier.pmid34500042en_US
dc.identifier.scopus2-s2.0-85114397737en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.mce.2021.111451
dc.identifier.urihttps://hdl.handle.net/11616/100190
dc.identifier.volume538en_US
dc.identifier.wosWOS:000702826300004en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Ireland Ltden_US
dc.relation.ispartofMolecular and Cellular Endocrinologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAsprosinen_US
dc.subjectGnRHen_US
dc.subjectFSHen_US
dc.subjectLHen_US
dc.subjectTestosteroneen_US
dc.subjectSpermen_US
dc.titleIntracerebroventricular asprosin administration strongly stimulates hypothalamic-pituitary-testicular axis in ratsen_US
dc.typeArticleen_US

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