The Metabolic Profile of Plasma During Epileptogenesis in a Rat Model of Lithium-Pilocarpine-Induced Temporal Lobe Epilepsy

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dc.contributor.authorAntmen, Fatma Merve
dc.contributor.authorMatpan, Emir
dc.contributor.authorDayanc, Ekin Dongel
dc.contributor.authorSavas, Eylem Ozge
dc.contributor.authorEken, Yunus
dc.contributor.authorAcar, Dilan
dc.contributor.authorAk, Alara
dc.date.accessioned2026-04-04T13:35:20Z
dc.date.available2026-04-04T13:35:20Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractTemporal lobe epilepsy (TLE) arises mostly because of an initial injury. Certain stimuli can make a normal brain prone to repeated, spontaneous seizures via a process called epileptogenesis. This study examined the plasma metabolomics profile in rats with the induced TLE to identify feasible biomarkers that can distinguish progression of epileptogenesis in three different time points and reveal the underlying mechanisms of epileptogenesis. Status epilepticus (SE) was induced by repetitive intraperitoneal injections of low-dose lithium chloride-pilocarpine hydrocholoride. Blood samples were collected 48 h, 1 week, and 6 weeks after SE, respectively. Plasma metabolites were analyzed by nuclear magnetic resonance (NMR) spectrometry. Statistical analysis was performed using MetaboAnalyst 6.0. An orthogonal partial least squares discriminant analysis (OPLS-DA) model was employed to represent variations between the TLE model groups and respective controls. Volcano plot analysis was used to identify key features, applying a fold-change criterion of 1.5 and a t-test threshold of 0.05. 48 h after SE, dimethyl sulfone (DMSO2) and creatinine levels were decreased, whereas glycine and creatine levels were increased. The only metabolite that changed 1 week after SE was pyruvic acid, which was increased compared to its control level. Lactic acid, pyruvic acid, and succinic acid levels were increased 6 weeks after SE. The identified metabolites were especially related to the tricarboxylic acid cycle and glycine, serine, and threonine metabolism. The results illustrate that distinct plasma metabolites can function as phase-specific biomarkers in TLE and reveal new insights into the mechanisms underlying SE.
dc.description.sponsorshipThe Scientific and Technological Research Council of Turkey-Directorate of Scientist Support Programs, 2244 Industrial Doctor of Philosophy (Ph.D.) Program (TUBITAK-BIDEB) 2244
dc.description.sponsorshipThe authors thank Emel Timucin for statistical suggestions.
dc.identifier.doi10.1007/s12035-025-04719-6
dc.identifier.endpage7483
dc.identifier.issn0893-7648
dc.identifier.issn1559-1182
dc.identifier.issue6
dc.identifier.orcid0009-0009-8600-4099
dc.identifier.orcid0000-0002-7199-5223
dc.identifier.orcid0000-0002-8814-7351
dc.identifier.orcid0000-0002-6175-897X
dc.identifier.orcid0000-0002-8053-7523
dc.identifier.pmid39904962
dc.identifier.scopus2-s2.0-85218016768
dc.identifier.scopusqualityQ1
dc.identifier.startpage7469
dc.identifier.urihttps://doi.org/10.1007/s12035-025-04719-6
dc.identifier.urihttps://hdl.handle.net/11616/109790
dc.identifier.volume62
dc.identifier.wosWOS:001412755500001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMolecular Neurobiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjectEpileptogenesis
dc.subjectNMR
dc.subjectEpilepsy
dc.subjectMetabolomics
dc.subjectPlasma
dc.subjectRat
dc.titleThe Metabolic Profile of Plasma During Epileptogenesis in a Rat Model of Lithium-Pilocarpine-Induced Temporal Lobe Epilepsy
dc.typeArticle

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