Sıçanlarda özefagus ve midede yaşa bağlı değişimlerin histomorfolojik açıdan incelenmesi
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Tarih
2013
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Öz: Amaç: Bu çalışmada sıçan özefagus ve midelerinde yaşa bağlı olarak gelişen morfolojik değişikliklerin ışık mikroskobik açıdan incelenmesi amaç- landı. Gereç ve Yöntem: Çalışmamızda 21 adet dişi Spraque-Dawley sıçan kullanıldı. Sıçanlar, Grup 1: 21 günlük sıçanlar, Grup 2: 2 aylık sıçanlar ve Grup 3: 19 aylık sıçanlar olmak üzere 3 gruba ayrıldı. Deney sonunda çıkartılan özefagus ve mide dokuları rutin histolojik doku takibinden sonra parafine gömüldü. Kesitler Hematoksilen-eozin ile boyanıp ışık mikroskobunda incelendi. Bulgular: Çalışmamızda özefagus epitel kalınlığı Grup 1’de ortalama 31.85±5.7 ?m, Grup 2’de 40.95±6.7 ?m, Grup 3’de 44.68±9,2 ?m olarak bulundu. Tunika muskularis kalınlığı Grup 1’de ortalama 128.9±23.1 ?m, Grup 2’de 207.8±38.5 ?m ve Grup 3’de 256.07±35.52 ?m olarak tespit edildi. Sıçanlarda özefagusa ait epitel ve tunika muskularis kalınlığı ölçüldüğünde yaşlanmaya bağlı olarak kalınlığın anlamlı derecede arttığı gözlendi (p<0.05). Çalışmamızda sıçanların mide tunika mukoza kalınlığı Grup 1’de ortalama 67.87±8.25 ?m, Grup 2’de 127.76±21.8 ?m ve Grup 3’de 181.23±35.1 ?m olarak bulundu. Tunika muskularis kalınlığı Grup 1’de 56.65±15.9 ?m, Grup 2’de 125.68±21.36 ?m ve Grup 3’de 174.07±22.5 ?m olarak tespit edildi. Sıçanlarda mideye ait tunika mukoza ve tunika muskularis kalınlığı ölçüldüğünde yaşlanmaya bağlı olarak kalınlığın anlamlı derecede arttığı gözlendi (p<0.05). Sonuç: Sıçanlarda özefagus ve mide morfolojisinin artan yaş ile değişim gösterdiği izlendi. Bu çalışmada elde edilen verilerin diğer morfolojik çalış- malara ışık tutacağı ve literatüre katkıda bulunacağı kanısını taşımaktayız.
Abstract: Objective: In this study, it was aimed that age-related morphological changes in rat esophagus and stomach was investigated with light microscopy. Materials and Methods: In this study, 21 female Sprage-Dawley rats were used. Rats were divided into three groups as follows. Group1: 21 days old, Group2: 2 month old and Group3: 19 month old. At the end of the examination esophagus and stomach tissues were removed.The sections stained wiht hematoxylin-eosin and evaluated light microscopically. Results: The mean thickness of esophagus epithelium was measured. It was 31.85±5.7 &#956;m in Group1, 40.95±6.7 &#956;m in Group2 and 44.68±9.2 &#956;m in Group3. The mean thickness of tunica muscularis in esophagus measured. It was 128.9±23.1 &#956;m in Group 1, 207.8±38,5 &#956;m in Group2 and 256.07±35.52 in Group3. We observed that the thickness of esophagus epithelium and tunica muscularis were significantly increased by age in all groups (p<0.05). Likewise, the mean thickness of tunica mucosa in stomach was measured. It was 67.87±8.25 &#956;m in Group1, 127.76±21.8 &#956;m in Group2 and 181.23±35.1 &#956;m in Group3. The mean thickness of tunika muscularis in stomach was measured. It was 56.65±15.9 &#956;m in Group1, 125.68±21.36&#956;m in Group2, 174.07±22.5 &#956;m in Group3. We observed that the thickness of tunica mucosa and tunica muscularis were significantly increased by age in all groups (p<0.05). Conclusion: The morfology of esophagus and stomach in rats were observed to change with increasing age. We believe that obtained in this study as age-related changes in rat esophagus and stomach may shed light on other morphological studies and that will contribute to the literature.
Abstract: Objective: In this study, it was aimed that age-related morphological changes in rat esophagus and stomach was investigated with light microscopy. Materials and Methods: In this study, 21 female Sprage-Dawley rats were used. Rats were divided into three groups as follows. Group1: 21 days old, Group2: 2 month old and Group3: 19 month old. At the end of the examination esophagus and stomach tissues were removed.The sections stained wiht hematoxylin-eosin and evaluated light microscopically. Results: The mean thickness of esophagus epithelium was measured. It was 31.85±5.7 &#956;m in Group1, 40.95±6.7 &#956;m in Group2 and 44.68±9.2 &#956;m in Group3. The mean thickness of tunica muscularis in esophagus measured. It was 128.9±23.1 &#956;m in Group 1, 207.8±38,5 &#956;m in Group2 and 256.07±35.52 in Group3. We observed that the thickness of esophagus epithelium and tunica muscularis were significantly increased by age in all groups (p<0.05). Likewise, the mean thickness of tunica mucosa in stomach was measured. It was 67.87±8.25 &#956;m in Group1, 127.76±21.8 &#956;m in Group2 and 181.23±35.1 &#956;m in Group3. The mean thickness of tunika muscularis in stomach was measured. It was 56.65±15.9 &#956;m in Group1, 125.68±21.36&#956;m in Group2, 174.07±22.5 &#956;m in Group3. We observed that the thickness of tunica mucosa and tunica muscularis were significantly increased by age in all groups (p<0.05). Conclusion: The morfology of esophagus and stomach in rats were observed to change with increasing age. We believe that obtained in this study as age-related changes in rat esophagus and stomach may shed light on other morphological studies and that will contribute to the literature.
Açıklama
Anahtar Kelimeler
Kaynak
Fırat Tıp Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
18
Sayı
2
Künye
TAŞLIDERE E,KURUŞ M,KAZANCI A,OTLU A (2013). Sıçanlarda özefagus ve midede yaşa bağlı değişimlerin histomorfolojik açıdan incelenmesi. Fırat Tıp Dergisi, 18(2), 75 - 82.
