Replication independent MCB gene induction and deoxyribonucleotide accumulation at G1 S in Saccharomyces cerevisiae
Yükleniyor...
Dosyalar
Tarih
2003
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
J Biol Chem
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In Saccharomyces cerevisiae, many genes encoding enzymes
involved in deoxyribonucleotide synthesis are expressed
preferentially near the G1/S boundary of the cell
cycle. The relationship between the induction of
deoxyribonucleotide-synthesizing genes, deoxyribonucleoside
triphosphate levels, and replication initiation
was investigated using factor-synchronized wildtype
yeast or dbf4 yeast that are temperature-sensitive
for replication initiation. Neither the timing nor extent
of gene induction was inhibited when factor-arrested
dbf4 cells were released into medium containing the
ribonucleotide reductase inhibitor hydroxyurea, which
blocks replication fork progression, or were released at
37 °C, which blocks replication origin firing. Thus, the
induction of deoxyribonucleotide-synthesizing genes at
G1/S was fully independent of DNA chain elongation or
initiation. Deoxyribonucleoside triphosphate levels increased
severalfold at G1/S in wild-type cells and in dbf4
mutants incubated at the non-permissive temperature.
Thus, deoxyribonucleoside triphosphate accumulation,
like the induction of deoxyribonucleotide-synthesizing
genes, was not dependent on replication initiation. Deoxyribonucleoside
triphosphate accumulation at G1/S
was suppressed in cells lacking Swi6, a transcription
factor required for normal cell cycle regulation of deoxyribonucleotide-synthesizing
genes. The results suggest
that cells use gene induction at G1/S as a mechanism
to pre-emptively, rather than reflexively, increase the
synthesis of DNA precursors to meet the demand of the
replication forks for deoxyribonucleotides.
Açıklama
J Biol Chem
Anahtar Kelimeler
Kaynak
J Biol Chem
WoS Q Değeri
Scopus Q Değeri
Cilt
278
Sayı
11
Künye
KOÇ, A., Linda, W., Chris, M., & Gary, M. (2003). Replication independent MCB gene induction and deoxyribonucleotide accumulation at G1 S in Saccharomyces cerevisiae . J Biol Chem, (278(11)), 9345–0.
