Replication independent MCB gene induction and deoxyribonucleotide accumulation at G1 S in Saccharomyces cerevisiae
| dc.authorid | 110769 | en_US |
| dc.contributor.author | Koç, Ahmet | |
| dc.contributor.author | Wheeler, Linda J. | |
| dc.contributor.author | Mathews, Christopher K. | |
| dc.contributor.author | Merrill, Gary F. | |
| dc.date.accessioned | 2017-06-19T11:48:35Z | |
| dc.date.available | 2017-06-19T11:48:35Z | |
| dc.date.issued | 2003 | |
| dc.department | İnönü Üniversitesi | en_US |
| dc.description | J Biol Chem | en_US |
| dc.description.abstract | In Saccharomyces cerevisiae, many genes encoding enzymes involved in deoxyribonucleotide synthesis are expressed preferentially near the G1/S boundary of the cell cycle. The relationship between the induction of deoxyribonucleotide-synthesizing genes, deoxyribonucleoside triphosphate levels, and replication initiation was investigated using factor-synchronized wildtype yeast or dbf4 yeast that are temperature-sensitive for replication initiation. Neither the timing nor extent of gene induction was inhibited when factor-arrested dbf4 cells were released into medium containing the ribonucleotide reductase inhibitor hydroxyurea, which blocks replication fork progression, or were released at 37 °C, which blocks replication origin firing. Thus, the induction of deoxyribonucleotide-synthesizing genes at G1/S was fully independent of DNA chain elongation or initiation. Deoxyribonucleoside triphosphate levels increased severalfold at G1/S in wild-type cells and in dbf4 mutants incubated at the non-permissive temperature. Thus, deoxyribonucleoside triphosphate accumulation, like the induction of deoxyribonucleotide-synthesizing genes, was not dependent on replication initiation. Deoxyribonucleoside triphosphate accumulation at G1/S was suppressed in cells lacking Swi6, a transcription factor required for normal cell cycle regulation of deoxyribonucleotide-synthesizing genes. The results suggest that cells use gene induction at G1/S as a mechanism to pre-emptively, rather than reflexively, increase the synthesis of DNA precursors to meet the demand of the replication forks for deoxyribonucleotides. | en_US |
| dc.identifier.citation | KOÇ, A., Linda, W., Chris, M., & Gary, M. (2003). Replication independent MCB gene induction and deoxyribonucleotide accumulation at G1 S in Saccharomyces cerevisiae . J Biol Chem, (278(11)), 9345–0. | en_US |
| dc.identifier.endpage | 0 | en_US |
| dc.identifier.issue | 11 | en_US |
| dc.identifier.startpage | 9345 | en_US |
| dc.identifier.uri | http://www.jbc.org/content/278/11/9345.full.pdf | |
| dc.identifier.uri | https://hdl.handle.net/11616/7111 | |
| dc.identifier.volume | 278 | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | J Biol Chem | en_US |
| dc.relation.ispartof | J Biol Chem | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.title | Replication independent MCB gene induction and deoxyribonucleotide accumulation at G1 S in Saccharomyces cerevisiae | en_US |
| dc.type | Article | en_US |
