Inhibition of Glomerular Mesangial Cell Proliferation by siPDGF-B- and siPDGFR-?-Containing Chitosan Nanoplexes

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dc.authoridSalva, Emine/0000-0002-1159-5850
dc.authoridŞALVA, EMINE/0000-0002-1159-5850
dc.authorwosidSalva, Emine/ABI-2766-2020
dc.authorwosid/AAD-1704-2020
dc.authorwosidŞALVA, EMINE/CAH-3062-2022
dc.contributor.authorSalva, Emine
dc.contributor.authorTuran, Suna Ozbas
dc.contributor.authorAkbuga, Julide
dc.date.accessioned2024-08-04T20:43:57Z
dc.date.available2024-08-04T20:43:57Z
dc.date.issued2017
dc.departmentİnönü Üniversitesien_US
dc.description.abstractMesangioproliferative glomerulonephritis is a disease that has a high incidence in humans. In this disease, the proliferation of glomerular mesangial cells and the production of extracellular matrix are important. In recent years, the RNAi technology has been widely used in the treatment of various diseases due to its capability to inhibit the gene expression with high specificity and targeting. The objective of this study was to decrease mesangial cell proliferation by knocking down PDGF-B and its receptor, PDGFR-beta. To be able to use small interfering RNAs (siRNAs) in the treatment of this disease successfully, it is necessary to develop appropriate delivery systems. Chitosan, which is a biopolymer, is used as a siRNA delivery system in kidney drug targeting. In order to deliver siRNA molecules targeted at PDGF-B and PDGFR-beta, chitosan/siRNA nanoplexes were prepared. The in vitro characterization, transfection studies, and knockdown efficiencies were studied in immortalized and primary rat mesangial cells. In addition, the effects of chitosan nanoplexes on mesangial cell proliferation and migration were investigated. After in vitro transfection, the PDGF-B and PDGFR-beta gene silencing efficiencies of PDGF-B and PDGFR-beta targeting siRNA-containing chitosan nanoplexes were 74 and 71% in immortalized rat mesangial cells and 66 and 62% in primary rat mesangial cells, respectively. siPDGF-B- and siPDGFR-beta-containing nanoplexes indicated a significant decrease in mesangial cell migration and proliferation. These results suggested that mesangial cell proliferation may be inhibited by silencing of the PDGF-B signaling pathway. Gene silencing approaches with chitosan-based gene delivery systems have promise for the efficient treatment of renal disease.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [113S202]en_US
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK, 113S202).en_US
dc.identifier.doi10.1208/s12249-016-0687-8
dc.identifier.endpage1042en_US
dc.identifier.issn1530-9932
dc.identifier.issue4en_US
dc.identifier.pmid27975193en_US
dc.identifier.scopus2-s2.0-85028245262en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1031en_US
dc.identifier.urihttps://doi.org/10.1208/s12249-016-0687-8
dc.identifier.urihttps://hdl.handle.net/11616/97944
dc.identifier.volume18en_US
dc.identifier.wosWOS:000404548700010en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofAaps Pharmscitechen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectchitosanen_US
dc.subjectglomerular mesangial cellen_US
dc.subjectPDGF-Ben_US
dc.subjectPDGFR-betaen_US
dc.subjectsiRNAen_US
dc.titleInhibition of Glomerular Mesangial Cell Proliferation by siPDGF-B- and siPDGFR-?-Containing Chitosan Nanoplexesen_US
dc.typeArticleen_US

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