Lactiplantibacillus Plantarum Postbiotics Suppress Salmonella Typhimurium Invasion and Modulate Innate Responses in Human Intestinal Epithelial Cells

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dc.contributor.authorCobur, Hazal
dc.contributor.authorLoker, Neriman
dc.contributor.authorDishan, Adalet
dc.contributor.authorVurmaz, Melike
dc.contributor.authorKaratepe, Pinar
dc.contributor.authorHizlisoy, Harun
dc.contributor.authorAkcay, Aytac
dc.date.accessioned2026-04-04T13:35:18Z
dc.date.available2026-04-04T13:35:18Z
dc.date.issued2025
dc.departmentİnönü Üniversitesi
dc.description.abstractPostbiotics derived from Lactiplantibacillus plantarum (LPP) exhibit promising potential as safe modulators of intestinal health. Here, the chemical, antimicrobial, and host-regulatory properties of LPP against Salmonella enterica serovar Typhimurium were investigated using a human intestinal epithelial (Caco-2) cell model. The LPP exhibited broad-spectrum antimicrobial activity against major foodborne pathogens, with minimal inhibitory concentrations ranging between 12.5 and 25 mg/mL. Treatment of Caco-2 cells with sub-MIC doses of LPP (6.25 mg/mL) significantly suppressed intracellular invasion of Salmonella (P < 0.05). Gene expression analysis revealed consistent downregulation of NF-kappa B gene expression following LPP presence with sublethal doses (P < 0.001), while no significant changes were detected in the expression levels of other key proinflammatory cytokines during the early phases (six and 12 h) of infection. A significant increase (P < 0.001) in apoptotic cell rates occurred at six h post-infection (PI), while no significant differences were observed at 12 h PI. The results revealed that LPP not only suppresses Salmonella invasion but also shows time-dependent modulation of inflammatory signaling and apoptotic responses in intestinal epithelial cells. These observed effects can be attributed to the bioactive compounds present in LPP, including organic acids, free amino acids, free fatty acids, polyphenols, and volatiles. The findings suggest that L. plantarum postbiotics can attenuate bacterial invasion and modulate innate immune response mechanisms without compromising host cell viability.
dc.description.sponsorshipTurkish Higher Education Council, Research Universities Support Program [TSAUE-2023-12220]
dc.description.sponsorshipThis research was supported by the Turkish Higher Education Council, Research Universities Support Program, with project number TSAUE-2023-12220 under the supervision of the Erciyes University Scientific Research Projects Coordination Unit.
dc.identifier.doi10.1007/s12602-025-10824-8
dc.identifier.issn1867-1306
dc.identifier.issn1867-1314
dc.identifier.orcid0000-0003-1178-3365
dc.identifier.pmid41182679
dc.identifier.scopus2-s2.0-105020883723
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1007/s12602-025-10824-8
dc.identifier.urihttps://hdl.handle.net/11616/109763
dc.identifier.wosWOS:001607776300001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofProbiotics and Antimicrobial Proteins
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250329
dc.subjectApoptosis
dc.subjectCaco-2 cell lines
dc.subjectHost-pathogen interactions
dc.subjectInnate response
dc.subjectSalmonella Typhimurium
dc.titleLactiplantibacillus Plantarum Postbiotics Suppress Salmonella Typhimurium Invasion and Modulate Innate Responses in Human Intestinal Epithelial Cells
dc.typeArticle

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