Catechin-Molecularly Imprinted Cryogel for Determination of Catechin in Red Wines by HPLC-DAD-Fluorescence Detector

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dc.authoridBuyuktuncel, Ebru/0000-0001-7269-6594
dc.authorwosidBuyuktuncel, Saliha Ebru/ABH-9343-2020
dc.contributor.authorBuyuktuncel, Ebru
dc.contributor.authorPorgali, Esra
dc.contributor.authorOzkara, Serpil
dc.date.accessioned2024-08-04T20:44:26Z
dc.date.available2024-08-04T20:44:26Z
dc.date.issued2018
dc.departmentİnönü Üniversitesien_US
dc.description.abstractA new molecularly imprinted polymer (MIP) was prepared by using catechin (C) as the template molecule. The polymer was characterized by swelling tests, scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), and Fourier transform infrared (FTIR) spectroscopy. The MIP with high recovery was selected as a solid-phase extraction (SPE) sorbent in this work. The standard solutions were directly applied onto the SPE cartridges following loading, washing, and elution procedures. A solution of the collected fractions was analyzed by high-performance liquid chromatography-diode-array detection (DAD) and fluorescence detector. The optimization of the method and validation was achieved on a C18 column (5 mu m, 250 x 4.6 mm) with methanol-water (35:65, v/v) mixture adjusted pH 2.5 as the mobile phase at a flow rate of 1 mL min(-1) at room temperature. The selectivity coefficient (k) of imprinted p(HEMA-MAH) cryogel was 5.1-fold that of non-imprinted cryogel. It showed good selectivity and affinity for C molecule. A comparison was made between the results obtained with the MIP cartridges and a traditional C18 reversed-phase cartridge. It was observed that 2.3 times higher recovery of C can be obtained on catechin-MIP cryogel. The results of the presented work showed that the prepared MIP can be used as SPE sorbent for extracting of C from red wines.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey [112T644]en_US
dc.description.sponsorshipThis research was financially supported by the Scientific and Technological Research Council of Turkey, as part of project no. 112T644.en_US
dc.identifier.doi10.1556/1326.2016.00193
dc.identifier.endpage61en_US
dc.identifier.issn1233-2356
dc.identifier.issn2083-5736
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85045920205en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage54en_US
dc.identifier.urihttps://doi.org/10.1556/1326.2016.00193
dc.identifier.urihttps://hdl.handle.net/11616/98251
dc.identifier.volume30en_US
dc.identifier.wosWOS:000428083500010en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherAkademiai Kiado Zrten_US
dc.relation.ispartofActa Chromatographicaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMolecularly imprinted polymeren_US
dc.subjectHPLC-DAD-fluorescence detectoren_US
dc.subjectcatechinen_US
dc.subjectred wineen_US
dc.titleCatechin-Molecularly Imprinted Cryogel for Determination of Catechin in Red Wines by HPLC-DAD-Fluorescence Detectoren_US
dc.typeArticleen_US

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