Novel Bacterial Cellulose Membrane to Reduce Fibrosis Following Trabeculectomy

Basit Öğe Kaydı
dc.authoridyazgan, serpil/0000-0001-6401-6376
dc.authoridAKPOLAT, Nusret/0000-0002-9138-2117
dc.authoridtekin, ishak ozel/0000-0002-9969-4254
dc.authorwosidyazgan, serpil/Z-1951-2019
dc.authorwosidAKPOLAT, Nusret/ABF-6986-2020
dc.contributor.authorYazgan, Serpil
dc.contributor.authorTekin, Ishak Ozel
dc.contributor.authorAkpolat, Nusret
dc.contributor.authorKoc, Ozhan
dc.date.accessioned2024-08-04T20:50:24Z
dc.date.available2024-08-04T20:50:24Z
dc.date.issued2021
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPurpose: The aim was to evaluate the effectiveness of bacterial cellulose membrane (BCM) in preventing fibrosis in trabeculectomy and the biocompatibility of BCM with conjunctiva and sclera. Materials and Methods: Twenty-one eyes of 21 adult rabbits underwent fornix-based trabeculectomy. Standard surgery was done to control group (CG, n = 7). Mitomycin-C (MMC) (0.3 mg/mL, 3 min) was applied to MMC group only (MMCG, n = 7). BCM (similar to 100 mu m thick, 10x 10 mm, single layer) was covered on the sclerotomy area before conjunctiva was closed in BCM group (BCMG, n = 7). Intraocular pressures (TOP) were measured before, and 7, 14, 28, and 45 days after surgery (IOP-POD7, POD14, POD28, POD45). The IOP decrease were expressed as DIOP%-POD7, DIOP%-POD14, DIOP%-POD28, and DIOP%-POD45. The rabbits were sacrificed on the 45th day. Conjunctival vessel number, degrees of fibrosis, total inflammation, foreign body reaction, inflammatory cell types (B cells, T cells, plasma cells), macrophages, bleb spaces and the expression of a-smooth muscle actin were studied using histopathology and immunohistochemistry techniques. The groups were compared using nonparametric tests. Results: There was no statistically significant difference between the groups regarding baseline IOP and DIOP%-POD7 (P > 0.05). While DIOP%-POD14, 28 and 45 were similar between BCMG and MMCG, they were significantly lower in CG (P < 0.05). The lowest conjunctival vessel number was detected in the MMCG but the difference was not significant. There was no difference between BCMG and CG with regard to the numbers of B cells, T cells, and macrophages, however, these cells were significantly lower in MMCG (P < 0.05). Five cases had mild and 2 cases had moderate foreign body reaction in the BCMG. There was mild to moderate inflammation in all BCM cases. While fibrosis and alpha-smooth muscle actin staining were higher in the CG (P < 0.001), they were minimal in the BCM and MMCGs. Conclusions: BCM showed good biocompatibility and provided better control of IOP with minimal fibrosis at the trabeculectomy site compared with the control group.en_US
dc.description.sponsorshipInonu University Scientific Research Project Unit [TCD-2020-2141]en_US
dc.description.sponsorshipThis study was funding by Inonu University Scientific Research Project Unit (Project ID: TCD-2020-2141).en_US
dc.identifier.doi10.1097/IJG.0000000000001907
dc.identifier.endpage1010en_US
dc.identifier.issn1057-0829
dc.identifier.issn1536-481X
dc.identifier.issue11en_US
dc.identifier.pmid34224487en_US
dc.identifier.scopus2-s2.0-85110183198en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1001en_US
dc.identifier.urihttps://doi.org/10.1097/IJG.0000000000001907
dc.identifier.urihttps://hdl.handle.net/11616/100044
dc.identifier.volume30en_US
dc.identifier.wosWOS:000756613100008en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherLippincott Williams & Wilkinsen_US
dc.relation.ispartofJournal of Glaucomaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbacterial cellulose membraneen_US
dc.subjectbiocompatibilityen_US
dc.subjectconjunctival fibrosisen_US
dc.subjectmitomycin-Cen_US
dc.subjecttrabeculectomyen_US
dc.titleNovel Bacterial Cellulose Membrane to Reduce Fibrosis Following Trabeculectomyen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu