Antioxidative effect of melatonin, ascorbic acid and N-acetylcysteine on caerulein-induced pancreatitis and associated liver injury in rats

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dc.authorscopusid6701663430
dc.authorscopusid55807973900
dc.authorscopusid6507504123
dc.authorscopusid6603280726
dc.authorscopusid35562819000
dc.contributor.authorEşrefo?lu M.
dc.contributor.authorGül M.
dc.contributor.authorAteş B.
dc.contributor.authorBatçio?lu K.
dc.contributor.authorSelimo?lu M.A.
dc.date.accessioned2024-08-04T19:59:39Z
dc.date.available2024-08-04T19:59:39Z
dc.date.issued2006
dc.departmentİnönü Üniversitesien_US
dc.description.abstractAim: To investigate the role of oxidative injury in pancreatitis-induced hepatic damage and the effect of antioxidant agents such as melatonin, ascorbic acid and N-acetyl cysteine on caerulein-induced pancreatitis and associated liver injury in rats. Methods: Thirty-eight female Wistar rats were used. Acute pancreatitis (AP) was induced by two i.p. injections of caerulein at 2-h intervals (at a total dose of 100 ?g/kg b.wt). The other two groups received additional melatonin (20 mg/kg b.wt) or an antioxidant mixture containing L(+)-ascorbic acid (14.3 mg/kb.wt.) and N-acetyl cysteine (181 mg/kg b.wt.) i.p. shortly before each injection of caerulein. The rats were sacrificed by decapitation 12 h after the last injection of caerulein. Pancreatic and hepatic oxidative stress markers were evaluated by changes in the amount of lipid peroxides measured as malondialdehyde (MDA) and changes in tissue antioxidant enzyme levels, catalase (CAT) and glutathione peroxidase (GPx). Histopathological examination was performed using scoring systems. Results: The degree of hepatic cell degeneration, intracellular vacuolization, vascular congestion, sinusoidal dilatation and inflammatory infiltration showed a significant difference between caerulein and caerulein + melatonin (P=0.001), and careulein and caerulein + L(+)-ascorbic acid + N-acetyl cysteine groups (P=0.002). The degree of aciner cell degeneration, pancreatic edema, intracellular vacuolization and inflammatory infiltration showed a significant difference between caerulein and caerulein + melatonin (P=0.004), and careulein and caerulein + L(+)-ascorbic acid +N-acetyl cysteine groups (P=0.002). Caerulein-induced pancreatic and liver damage was accompanied with a significant increase in tissue MDA levels (P=0.01, P= 0.003, respectively) whereas a significant decrease in CAT (P=0.002, P=0.003, respectively) and GPx activities (P=0.002, P=0.03, respectively). Melatonin and L(+)-ascorbic acid + N-acetyl cysteine administration significantly decreased MDA levels in pancreas (P=0.03, P=0.002, respectively) and liver (P=0.007, P=0.01, respectively). Administration of these agents increased pancreatic and hepatic CAT and GPx activities. Melatonin significantly increased pancreatic and hepatic CAT (P=0.002, P=0.001, respectively) and GPx activities (P=0.002, P=0.001). Additionally, L(+)-ascorbic acid+N-acetyl cysteine significantly increased pancreatic GPx (P=0.002) and hepatic CAT and GPx activities (P= 0.001, P= 0.007, respectively). Conclusion: Oxidative injury plays an important role not only in the pathogenesis of AP but also in pancreatitis-induced hepatic damage. Antioxidant agents such as melatonin and ascorbic acid+N-acetyl cysteine, are capable of limiting pancreatic and hepatic damage produced during AP via restoring tissue antioxidant enzyme activities. © 2006 The WJG Press. All rights reserved.en_US
dc.identifier.doi10.3748/wjg.v12.i2.259
dc.identifier.endpage264en_US
dc.identifier.issn1007-9327
dc.identifier.issue2en_US
dc.identifier.pmid16482627en_US
dc.identifier.scopus2-s2.0-33644787377en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage259en_US
dc.identifier.urihttps://doi.org/10.3748/wjg.v12.i2.259
dc.identifier.urihttps://hdl.handle.net/11616/90793
dc.identifier.volume12en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherBaishideng Publishing Group Coen_US
dc.relation.ispartofWorld Journal of Gastroenterologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCaeruleinen_US
dc.subjectLiveren_US
dc.subjectMelatoninen_US
dc.subjectOxidative stressen_US
dc.subjectPancreatitisen_US
dc.titleAntioxidative effect of melatonin, ascorbic acid and N-acetylcysteine on caerulein-induced pancreatitis and associated liver injury in ratsen_US
dc.typeArticleen_US

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