Enhancement of enzyme activity by laser-induced energy propulsion of upconverting nanoparticles under near-infrared light: A comprehensive methodology for in vitro and in vivo applications

Basit Öğe Kaydı
dc.contributor.authorAtes, Burhan
dc.contributor.authorUlu, Ahmet
dc.contributor.authorAsilturk, Meltem
dc.contributor.authorNoma, Samir Abbas Ali
dc.contributor.authorTopel, Seda Demirel
dc.contributor.authorDik, Gamze
dc.contributor.authorOzhan, Onural
dc.date.accessioned2024-08-04T20:55:00Z
dc.date.available2024-08-04T20:55:00Z
dc.date.issued2024
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIf the appropriate immobilization method and carrier support are not selected, partial decreases in the activity of enzymes may occur after immobilization. Herein, to overcome this challenge, an excitation mechanism that enables energy transfer was proposed. Modified upconverting nanoparticles (UCNPs) were constructed and the important role of near-infrared (NIR) excitation in enhancing the catalytic activity of the enzyme was demonstrated. For this purpose, UCNPs were first synthesized via the hydrothermal method, functionalized with isocyanate groups, and then, PEG-L-ASNase was immobilized via covalent binding. UCNPs with and without PEG-LASNase were extensively characterized by different methods. These supports had immobilization yield and activity efficiency of >96 % and 78 %, respectively. Moreover, immobilized enzymes exhibited improved pH, thermal, and storage stability. In addition, they retained >65 % of their initial activity even after 20 catalytic cycles. Biochemical and histological findings did not indicate a trend of toxicity in rats due to UCNPs. Most importantly, PEG-L-ASNase activity was triggered approximately 5- and 2-fold under in vitro and in vivo conditions, respectively. Overall, it is anticipated that this pioneering work will shed new light on the realistic and promising usage of NIR-excited UCNPs for the immobilization of enzymes in expensive and extensive applications.en_US
dc.description.sponsorshipThe 2515-COST (European Cooperation in Science and Technology of the Scientific and Technological Research Council of Turkiye; [119Z962]; [CA17126]en_US
dc.description.sponsorshipThe authors gratefully acknowledge financial support from 2515-COST (European Cooperation in Science and Technology (TUMIEE-COST Action CA17126) ) of the Scientific and Technological Research Council of Turkiye (119Z962) . Busra Bakar is a 100/2000 the Council of Higher Education PhD Scholar Biomaterial and Tissue Engineering subdivision.en_US
dc.identifier.doi10.1016/j.ijbiomac.2024.129343
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid38242401en_US
dc.identifier.scopus2-s2.0-85183315524en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2024.129343
dc.identifier.urihttps://hdl.handle.net/11616/101780
dc.identifier.volume260en_US
dc.identifier.wosWOS:001174291500001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Journal of Biological Macromoleculesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEnzyme immobilizationen_US
dc.subjectPEG-L-asparaginaseen_US
dc.subjectUpconverting nanoparticles (UCNPs)en_US
dc.subjectNear-infrared (NIR) excitationen_US
dc.subjectTriggered activityen_US
dc.titleEnhancement of enzyme activity by laser-induced energy propulsion of upconverting nanoparticles under near-infrared light: A comprehensive methodology for in vitro and in vivo applicationsen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu