VIM-1, VIM-2, and GES-5 Carbapenemases Among Pseudomonas aeruginosa Isolates at a Tertiary Hospital in Istanbul, Turkey

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dc.authoridOTLU, BARIS/0000-0002-6220-0521
dc.authoridaktas, elif/0000-0003-3087-5425
dc.authorwosidBulut, Emin/AAY-8628-2021
dc.authorwosidaktas, elif/ACL-9792-2022
dc.authorwosidOTLU, BARIS/ABI-5532-2020
dc.contributor.authorMalkocoglu, Guelsah
dc.contributor.authorAktas, Elif
dc.contributor.authorBayraktar, Banu
dc.contributor.authorOtlu, Baris
dc.contributor.authorBulut, Mehmet Emin
dc.date.accessioned2024-08-04T20:43:05Z
dc.date.available2024-08-04T20:43:05Z
dc.date.issued2017
dc.departmentİnönü Üniversitesien_US
dc.description.abstractWorldwide increase in carbapenem resistance and transferable carbapenemases are significant challenges in treatment of Pseudomonas aeruginosa infections. In this study, investigation of carbapenemase production in carbapenem-resistant P. aeruginosa isolates recovered from clinical specimens in a tertiary hospital was aimed. A total of 84 carbapenem-resistant P. aeruginosa isolates were examined. Carbapenem inactivation method (CIM) was used for phenotypic detection of carbapenemase production. The existence of bla(KPC), bla(NDM), bla(IMP), bla(VIM), bla(OXA-48), and bla(GES) genes was investigated by polymerase chain reaction (PCR). Subtypes of the detected genes were identified by sequence analysis. Arbitrarily primed PCR (AP-PCR) was performed to evaluate the clonal relationship among the isolates. The presence of high-risk clones in carbapenemase producers was investigated by Matrix-assisted laser desorption/ionizationtime of flight mass spectrometry (MALDI-TOF MS). Three isolates (3.5%) were identified as carbapenemase producers by CIM tests, while PCR tests demonstrated three isolates carrying carbapenemase genes as well. bla(VIM) gene was found in two isolates and bla(GES) gene was found in one isolate. Sequence analysis demonstrated that the carbapenemases were VIM-1, VIM-2, and GES-5. AP-PCR yielded high clonal diversity among the isolates. According to MALDI-TOF MS analysis, none of the carbapenemase-producing strains belonged to the high-risk clones. In conclusion, the presence of VIM-1, VIM-2, and GES-5 type carbapenemases in P. aeruginosa isolates was demonstrated for the first time in our hospital, GES-5 being reported for the second time in Turkey. Our results will lead strategies for controlling the spread of carbapenemases and contribute to epidemiological data from Turkey.en_US
dc.identifier.doi10.1089/mdr.2016.0012
dc.identifier.endpage334en_US
dc.identifier.issn1076-6294
dc.identifier.issn1931-8448
dc.identifier.issue3en_US
dc.identifier.pmid27326514en_US
dc.identifier.scopus2-s2.0-85017154605en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage328en_US
dc.identifier.urihttps://doi.org/10.1089/mdr.2016.0012
dc.identifier.urihttps://hdl.handle.net/11616/97778
dc.identifier.volume23en_US
dc.identifier.wosWOS:000398818000009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMary Ann Liebert, Incen_US
dc.relation.ispartofMicrobial Drug Resistanceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectcarbapenemaseen_US
dc.subjectVIM-1en_US
dc.subjectVIM-2en_US
dc.subjectGES-5en_US
dc.subjectTurkeyen_US
dc.titleVIM-1, VIM-2, and GES-5 Carbapenemases Among Pseudomonas aeruginosa Isolates at a Tertiary Hospital in Istanbul, Turkeyen_US
dc.typeArticleen_US

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