Assessment of chronological lifespan dependent molecular damages in yeast lacking mitochondrial antioxidant genes

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dc.authorid190238en_US
dc.authorid110769en_US
dc.contributor.authorDemir, Ayşe Banu
dc.contributor.authorKoç, Ahmet
dc.date.accessioned2017-06-11T13:16:29Z
dc.date.available2017-06-11T13:16:29Z
dc.date.issued2010
dc.departmentİnönü Üniversitesien_US
dc.descriptionBiochemical and Biophysical Research Communications 400 (2010) 106–110en_US
dc.description.abstractThe free radical theory of aging states that oxidative damage to biomolecules causes aging and that antioxidants neutralize free radicals and thus decelerate aging. Mitochondria produce most of the reactive oxygen species, but at the same time have many antioxidant enzymes providing protection from these oxidants. Expecting that cells without mitochondrial antioxidant genes would accumulate higher levels of oxidative damage and, therefore, will have a shorter lifespan, we analyzed oxidative damages to biomolecules in young and chronologically aged mutants lacking the mitochondrial antioxidant genes: GRX2, CCP1, SOD1, GLO4, TRR2, TRX3, CCS1, SOD2, GRX5, and PRX1. Among these mutants, ccp1D, trx3D, grx5D, prx1D, mutants were sensitive to diamide, and ccs1D and sod2D were sensitive to both diamide and menadione. Most of the mutants were less viable in stationary phase. Chronologically aged cells produced higher amount of superoxide radical and accumulated higher levels of oxidative damages. Even though our results support the findings that old cells harbor higher amount of molecular damages, no significant difference was observed between wild type and mutant cells in terms of their damage content.en_US
dc.identifier.citationDemir, A. B., & Koç, A. (2010). Assessment Of Chronological Lifespan Dependent Molecular Damages İn Yeast Lacking Mitochondrial Antioxidant Genes. Biochemical And Biophysical Research Communications, 400(1), 106–110.en_US
dc.identifier.doi10.1016/j.bbrc.2010.08.019en_US
dc.identifier.endpage110en_US
dc.identifier.issue1en_US
dc.identifier.startpage106en_US
dc.identifier.urihttp://linkinghub.elsevier.com/retrieve/pii/S0006291X10015056
dc.identifier.urihttps://hdl.handle.net/11616/7083
dc.identifier.volume400en_US
dc.language.isoenen_US
dc.publisherBiochemical and Biophysical Research Communicationsen_US
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectChronological agingen_US
dc.subjectMolecular damageen_US
dc.subjectMitochondriaen_US
dc.subjectAntioxidant geneen_US
dc.subjectOxidative stressen_US
dc.titleAssessment of chronological lifespan dependent molecular damages in yeast lacking mitochondrial antioxidant genesen_US
dc.typeArticleen_US

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