TRPM2 mediates distruption of autophagy machinery and correlates with the grade level in prostate cancer

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dc.authoridÖZERCAN, ibrahim Hanifi/0000-0002-8781-8838
dc.authoridTektemur, Nalan Kaya/0000-0001-8880-4932
dc.authoridkuloğlu, tuncay/0000-0001-9874-3838
dc.authoridTekin, Suat/0000-0002-2757-1802
dc.authoridTektemur, Ahmet/0000-0002-2476-0413
dc.authorwosidÖZERCAN, ibrahim Hanifi/W-7883-2018
dc.authorwosidTekin, Suat/IZD-9868-2023
dc.authorwosidTekin, Suat/AAG-1440-2021
dc.authorwosidTektemur, Ahmet/V-8755-2018
dc.authorwosidTektemur, Nalan Kaya/W-6712-2018
dc.authorwosidkuloğlu, tuncay/W-2310-2018
dc.authorwosidTekin, Suat/KEI-2266-2024
dc.contributor.authorTektemur, Ahmet
dc.contributor.authorOzaydin, Seda
dc.contributor.authorOnalan, Ebru Etem
dc.contributor.authorKaya, Nalan
dc.contributor.authorKuloglu, Tuncay
dc.contributor.authorOzercan, Ibrahim Hanifi
dc.contributor.authorTekin, Suat
dc.date.accessioned2024-08-04T20:45:50Z
dc.date.available2024-08-04T20:45:50Z
dc.date.issued2019
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPurposeTransient receptor potential melastatin 2 (TRPM2), a calcium-permeable ion channel, is shown as a prognostic marker candidate in prostate cancer (PCa) and an important regulator of autophagy. We aimed to determine the changes in TRPM2 and autophagic-apoptotic gene expression levels in human prostate adenocarcinomas, and to investigate the affect of TRPM2 on autophagic pathways in PC-3 cell line.MethodsHuman prostate tissues were classified considering the grade levels and were divided into the control, BPH, and grade 1-5 groups. mRNA expression levels of genes were determined by qPCR. In addition, TRPM2 was evaluated immunohistochemically for each group. In PC-3 cell line, TRPM2 was silenced through siRNA transfection, and autophagy induction was analyzed by acridine orange (AO) staining.ResultsThe qPCR and immunoreactivity results showed that the increased TRPM2 expression levels in human PCa samples were paralleled with higher grade levels. The autophagic-apoptotic gene expressions showed high variability in different grade levels. Also, silencing TRPM2 in PC-3 cells altered autophagic gene expressions and caused autophagy induction according to the AO staining results.ConclusionWe showed that the autophagy-TRPM2 association may take place in the molecular basis of PCa and accordingly this connection may be targeted as a new therapeutic approach in PCa.en_US
dc.description.sponsorshipFirat University Scientific Research Projects Unit [TF.14.37]en_US
dc.description.sponsorshipThis project was supported by the Firat University Scientific Research Projects Unit (Project No: TF.14.37).en_US
dc.identifier.doi10.1007/s00432-019-02898-z
dc.identifier.endpage1311en_US
dc.identifier.issn0171-5216
dc.identifier.issn1432-1335
dc.identifier.issue5en_US
dc.identifier.pmid30888515en_US
dc.identifier.scopus2-s2.0-85063086931en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1297en_US
dc.identifier.urihttps://doi.org/10.1007/s00432-019-02898-z
dc.identifier.urihttps://hdl.handle.net/11616/98726
dc.identifier.volume145en_US
dc.identifier.wosWOS:000465605800018en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal of Cancer Research and Clinical Oncologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectProstate canceren_US
dc.subjectIon channelsen_US
dc.subjectTransient receptor potential melastatin 2 (TRPM2)en_US
dc.subjectAutophagyen_US
dc.subjectGene expressionen_US
dc.titleTRPM2 mediates distruption of autophagy machinery and correlates with the grade level in prostate canceren_US
dc.typeArticleen_US

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