Immobilization of Xylanase onto Starch Nanoparticles: A Reusable and Robust Nanobiocatalyst for Juice Clarification

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dc.authoridDik, Gamze/0000-0003-4798-8127
dc.authoridAtes, Burhan/0000-0001-6080-229X
dc.authoridKoytepe, Suleyman/0000-0002-4788-278X
dc.contributor.authorDik, Gamze
dc.contributor.authorBakar, Busra
dc.contributor.authorUlu, Ahmet
dc.contributor.authorKoytepe, Sueleyman
dc.contributor.authorAtes, Burhan
dc.date.accessioned2024-08-04T20:54:45Z
dc.date.available2024-08-04T20:54:45Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractNanoparticles (NPs) have attracted interest recently as a promising support for the immobilization of enzymes.Xylanase (Xyl) has been utilized in the food industry for several purposes. To improve its stability and reusability,starch NPs (SNPs)are synthesized and assessed for Xyl immobilization for the first time. . The characterization results reveal that SNPs are successfully synthesized and Xyl@SNPs are effective as the immobilization carrier. The Xyl is immobilized on SNPs under optimized conditions leading to 91% immobilization efficiency. A shift in optimum pH (from 6.0 to 8.0) and temperature (from 70 to 60 degrees C) of Xyl is observed after immobilization. The Xyl@SNPs possess improve pH stability and thermal stability as well as operational stability. The Xyl@SNPs are easily reutilized and the residual activity is still higher than 62% after seven times. More importantly, the Xyl@SNPs have a lower Km value and enhance affinity for the substrate compared to the free Xyl. Additionally, the clarity of orange juice is increased by 76.0% by using Xyl@SNPs after 2 h incubation at 60 degrees C, pH 8.0. These positive results show promising support for the Xyl immobilization of SNPs, confirming that this immobilized enzyme-based strategy is an effective technique for rapid clarification of targeted juices.en_US
dc.description.sponsorshipCouncil of Higher Education (CoHE) PhD Scholarship in Biomaterial and Tissue Engineering subdivision [100/2000]; Scientific Research Projects Unit of Inonu University [FBG-2021-2731, FOA-2021-2393]; Council of Higher Education (CoHE) [100/2000]en_US
dc.description.sponsorshipThis investigation was financially supported by the Scientific Research Projects Unit of Inonu University (Project Number: FBG-2021-2731 and FOA-2021-2393). Busra Bakar is a 100/2000 the Council of Higher Education (CoHE) PhD Scholarship in Biomaterial and Tissue Engineering subdivision.en_US
dc.identifier.doi10.1002/star.202300130
dc.identifier.issn0038-9056
dc.identifier.issn1521-379X
dc.identifier.scopus2-s2.0-85172766399en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1002/star.202300130
dc.identifier.urihttps://hdl.handle.net/11616/101597
dc.identifier.wosWOS:001075383900001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofStarch-Starkeen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectenzyme immobilizationen_US
dc.subjectjuice clarificationen_US
dc.subjectstarch nanoparticlesen_US
dc.subjectxylanaseen_US
dc.subjectimproved stabilityen_US
dc.titleImmobilization of Xylanase onto Starch Nanoparticles: A Reusable and Robust Nanobiocatalyst for Juice Clarificationen_US
dc.typeArticleen_US

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