Characterization, in vitro release, and antioxidant activity of glutenin hydrolysate encapsulated in liposome-loaded uni-axial and co-axial electrospun fibers

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dc.contributor.authorKalintas Caglar, Nagihan
dc.contributor.authorCaglar, Ahmet Furkan
dc.contributor.authorBozkurt, Fatih
dc.contributor.authorIzciler, Feyzanur
dc.contributor.authorSagdic, Osman
dc.contributor.authorKarakas, Canan Yagmur
dc.contributor.authorKaradag, Ayse
dc.date.accessioned2026-04-04T13:37:37Z
dc.date.available2026-04-04T13:37:37Z
dc.date.issued2026
dc.departmentİnönü Üniversitesi
dc.description.abstractBACKGROUND Bioactive peptides derived from protein hydrolysates provide various health benefits; however, their practical application is limited by low gastrointestinal stability, enzymatic degradation, and poor intestinal absorption. Overcoming these challenges remains a key bottleneck for oral peptide delivery. This study aimed to develop and systematically compare uni-axial and co-axial electrospun pullulan/carboxymethylcellulose fibers incorporating liposome-encapsulated glutenin hydrolysate (GH) to enhance its stability, mucoadhesion, and controlled release along the gastrointestinal system.RESULTS GH (7.5 mg mL-1) was encapsulated into lecithin-phytosterol (1:0.5, w/w) liposomes, yielding an average size of 76 nm and an encapsulation efficiency of 57.52%. These liposomes were successfully embedded into nanofibers, showing homogeneous distribution and GH loading efficiencies of 61.04-85.22%. Compared with free GH, liposomal systems preserved the antioxidant activity (ABTS and FRAP values) of GH during gastrointestinal digestion, while the non-hybrid formulation demonstrated reduced preservation. Liposome-loaded nanofibers exhibited markedly lower GH release under gastric conditions (21.05-25.85%) than free-GH fibers (42.69%), while co-axial fibers provided the most sustained intestinal release. Additionally, liposomal incorporation significantly enhanced mucoadhesive properties.CONCLUSION The hybrid liposome-nanofiber approach integrates protective and controlled-delivery mechanisms, resulting in enhanced preservation of antioxidant activity and sustained release compared with conventional fibers. This food-grade strategy shows strong potential for oral delivery of bioactive peptides in functional food and nutraceutical applications requiring gastrointestinal stability.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [122O666]; The 2211-A National PhD Scholarship Programs of TUBITAK; TUBITAK
dc.description.sponsorshipThis study was supported by Scientific and Technological Research Council of Turkey (TUBITAK) under the Grant Number 122O666. Nagihan Kalintas Caglar was also supported by the 2211-A National PhD Scholarship Programs of TUBITAK. The authors thank to TUBITAK for their supports. The graphical abstract was created by BIORENDER.
dc.identifier.doi10.1002/jsfa.70570
dc.identifier.issn0022-5142
dc.identifier.issn1097-0010
dc.identifier.orcid0000-0002-7700-5307
dc.identifier.pmid41813609
dc.identifier.scopus2-s2.0-105032553572
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1002/jsfa.70570
dc.identifier.urihttps://hdl.handle.net/11616/109951
dc.identifier.wosWOS:001711601100001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of the Science of Food and Agriculture
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250329
dc.subjectdigestion
dc.subjectelectrospinning
dc.subjectmucoadhesion
dc.subjectpeptide
dc.titleCharacterization, in vitro release, and antioxidant activity of glutenin hydrolysate encapsulated in liposome-loaded uni-axial and co-axial electrospun fibers
dc.typeArticle

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