Periodontal hastalığın patogenezinde Hippo sinyal yolağı proteinlerinin rolünün incelenmesi
Küçük Resim Yok
Tarih
2025
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
İnönü Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Hippo sinyal yolağı hücre farklılaşması ve ölümü gibi birçok önemli olayda görev almaktadır. Bu çalışmanın amacı farklı periodontal duruma sahip olan bireylerin dişeti oluğu sıvısı (DOS) ve dişeti dokusunda Hippo sinyal yolağı proteinlerinden YAP, TAZ, MST1, TEAD, MAP4K4 ve LATS1 ve apoptoz ile ilişki BCL-2 ve kemik metabolizması ile ilişkili ALP'nin seviyelerinin karşılaştırılmasıdır. Materyal ve Metot: Sistemik olarak sağlıklı 75 katılımcı periodontal sağlıklı (n=25), gingivitis (n=25) ve evre 3 derece B periodontitis (n=25) olmak üzere 3 gruba ayrılmış ve DOS örnekleri toplanmıştır. Sistemik olarak sağlıklı 60 katılımcı ise eşit sayıda ve aynı periodontal sınıflamalarla ayrılmış, hastalardan dişeti dokusu toplanmıştır. YAP, TAZ, MST1, LATS1, MAP4K4, TEAD, ALP ve BCL-2 seviyeleri DOS'ta ELISA ile dişetindeki gen ekspresyon seviyeleri ise RT-qPCR analizi ile belirlenmiştir. Bulgular: YAP ve TEAD DOS total miktarı ile YAP, TAZ, MST1, TEAD, LATS1, MAP4K4, ALP ve BCL-2 DOS konsantrasyonları periodontitis grubunda sağlıklı kontrollere göre azalmış, ALP DOS total miktarı ise artmış olarak bulunmuştur (p<0.05) Periodontitis grubunda sağlıklı kontrollere göre YAP ve TAZ'ın gen ekspresyonunun aşağı yönde regüle olduğu gözlenirken, TEAD, MST1, LATS1, MAP4K4, ALP ve BCL-2'nin ise yukarı yönlü regüle olduğu saptanmıştır (p<0.05). YAP ve TAZ gen ekspresyonu ile ALP arasında pozitif bir korelasyon saptanmıştır. Sonuç: Hippo sinyal yolağı ile ilişkili yapılar DOS ve dişetinde farklı etkiler göstererek periodontal hastalığın patogenezinde rol oynayabilir. Anahtar kelimeler: Hippo sinyal yolağı, ALP, BCL-2, periodontal inflamasyon, DOS
Objective: The Hippo signaling pathway is involved in many crucial processes, such as cell differentiation and death. This study aims to compare the levels of Hippo signaling pathway proteins (YAP, TAZ, MST1, TEAD, MAP4K4, and LATS1), apoptosis-related BCL-2, and bone metabolism-related ALP in gingival crevicular fluid (GCF) and gingival tissue in individuals with different periodontal conditions. Material and Method: A total of 75 systemically healthy participants were divided into three groups: periodontal healthy (n=25), gingivitis (n=25), and stage 3 grade B periodontitis (n=25). GCF samples were collected from these participants. Another 60 systemically healthy participants were also divided into equal numbers with the same periodontal classifications, and gingival tissue was collected from these patients. The levels of YAP, TAZ, MST1, LATS1, MAP4K4, TEAD, ALP, and BCL-2 in GCF were determined by ELISA, while gene expression levels in gingival tissue were detected by RT-qPCR. Results: The total amount of YAP and TEAD in GCF and the concentrations of YAP, TAZ, MST1, TEAD, LATS1, MAP4K4, ALP, and BCL-2 were lower in the periodontitis group compared to the healthy controls (p<0.05). However, ALP total levels in GCF were higher. Gene expression of YAP and TAZ was downregulated, while TEAD, MST1, LATS1, MAP4K4, ALP, and BCL-2 were upregulated in the periodontitis group (p<0.05). A positive correlation between YAP and TAZ gene expression and ALP was observed. Conclusions: Hippo signaling pathway may play a role in the pathogenesis of periodontal disease by showing different effects in GCF and gingival tissue. Keywords: Hippo signaling pathway, ALP, BCL-2, periodontal inflammation, GCF
Objective: The Hippo signaling pathway is involved in many crucial processes, such as cell differentiation and death. This study aims to compare the levels of Hippo signaling pathway proteins (YAP, TAZ, MST1, TEAD, MAP4K4, and LATS1), apoptosis-related BCL-2, and bone metabolism-related ALP in gingival crevicular fluid (GCF) and gingival tissue in individuals with different periodontal conditions. Material and Method: A total of 75 systemically healthy participants were divided into three groups: periodontal healthy (n=25), gingivitis (n=25), and stage 3 grade B periodontitis (n=25). GCF samples were collected from these participants. Another 60 systemically healthy participants were also divided into equal numbers with the same periodontal classifications, and gingival tissue was collected from these patients. The levels of YAP, TAZ, MST1, LATS1, MAP4K4, TEAD, ALP, and BCL-2 in GCF were determined by ELISA, while gene expression levels in gingival tissue were detected by RT-qPCR. Results: The total amount of YAP and TEAD in GCF and the concentrations of YAP, TAZ, MST1, TEAD, LATS1, MAP4K4, ALP, and BCL-2 were lower in the periodontitis group compared to the healthy controls (p<0.05). However, ALP total levels in GCF were higher. Gene expression of YAP and TAZ was downregulated, while TEAD, MST1, LATS1, MAP4K4, ALP, and BCL-2 were upregulated in the periodontitis group (p<0.05). A positive correlation between YAP and TAZ gene expression and ALP was observed. Conclusions: Hippo signaling pathway may play a role in the pathogenesis of periodontal disease by showing different effects in GCF and gingival tissue. Keywords: Hippo signaling pathway, ALP, BCL-2, periodontal inflammation, GCF
Açıklama
Anahtar Kelimeler
Diş Hekimliği, Dentistry
