Differential expression of miRNAs related to autophagy pathway in tissue and serum samples of colorectal cancer patients

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dc.authoridYilmaz, Umit/0000-0003-0248-3483
dc.authoridYilmaz, Nesibe/0000-0002-5527-8507
dc.authorwosidYilmaz, Umit/JFA-5784-2023
dc.authorwosidYilmaz, Nesibe/JFK-5834-2023
dc.contributor.authorUmit, Yilmaz
dc.contributor.authorNesibe, Yilmaz
dc.contributor.authorKevser, Tanbek
dc.contributor.authorArzu, Ergen
dc.contributor.authorNihat, Aksakal
dc.contributor.authorUmit, Zeybek
dc.date.accessioned2024-08-04T20:52:14Z
dc.date.available2024-08-04T20:52:14Z
dc.date.issued2022
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBACKGROUND: This study was aimed to investigate the relationship of miR-17-5p, miR-30b, miR-30d, miR-216a and miR-216b associated with autophagy gene beclin 1, and beclin 1 gene with colorectal cancer (CRC).MATERIALS AND METHODS: Forty-seven patients with CRC and 50 healthy individuals with no cancer history were included in this study. In the serum, tumor and non-tumoral tissue samples of the CRC patients, and in the serum samples of the healthy subjects, expression levels of miRNAs were detected by qRT-PCR. The beclin 1 gene expression levels were determined by qRT-PCR, and protein levels were determined by Western blot method in tumor and non-tumor tissue samples of the patients.RESULTS: The miR-17-5p and miR-30d expressions were found to be higher in tumor tissue as compared to patient non-tumor tissues, while expressions of beclin-1, miR-30b and miR-216a were found to be lower. In addition, the beclin-1 protein levels were significantly decreased in the tumor tissue as compared to those in the patient non-tumor tissues. The miR-30d expression was significantly reduced in the serum of the patients when the serum samples of CRC patients and healthy controls were compared.CONCLUSION: The beclin 1 gene may play a role as a tumor suppressor in CRC. Moreover, these miRNAs cannot be used as highly reliable biomarkers in serum for CRC diagnosis (Tab. 2, Fig. 6, Ref. 46). Text in PDF www.elis.sken_US
dc.description.sponsorshipResearch Fund of Istanbul University [29126]; Scientific and Technological Research Council of Turkey (TUEBITAK) [215S540]en_US
dc.description.sponsorshipThe present work was supported by the Research Fund of Istanbul University [Project No: 29126] and The Scientific and Technological Research Council of Turkey (TUEBITAK) [Project No: 215S540].en_US
dc.identifier.doi10.4149/BLL_2022_102
dc.identifier.endpage640en_US
dc.identifier.issn0006-9248
dc.identifier.issn1336-0345
dc.identifier.issue9en_US
dc.identifier.pmid36039881en_US
dc.identifier.scopus2-s2.0-85135831644en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage634en_US
dc.identifier.urihttps://doi.org/10.4149/BLL_2022_102
dc.identifier.urihttps://hdl.handle.net/11616/100839
dc.identifier.volume123en_US
dc.identifier.wosWOS:000903730000005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAepress Sroen_US
dc.relation.ispartofBratislava Medical Journal-Bratislavske Lekarske Listyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectcolorectal canceren_US
dc.subjectautophagyen_US
dc.subjectbeclin-1en_US
dc.subjectmiRNAen_US
dc.subjectbiomarkeren_US
dc.titleDifferential expression of miRNAs related to autophagy pathway in tissue and serum samples of colorectal cancer patientsen_US
dc.typeArticleen_US

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