Simultaneous detection of isoniazid, rifampin, and ethambutol resistance of Mycobacterium tuberculosis by a single multiplex allele-specific polymerase chain reaction (PCR) assay

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dc.authoridGünal, Selami/0000-0002-4752-5176
dc.authoridFoxman, Betsy/0000-0001-6682-238X
dc.authoridDURMAZ, RIZA/0000-0001-6561-778X
dc.authorwosidGünal, Selami/AAA-7246-2021
dc.authorwosidFoxman, Betsy/E-1836-2015
dc.authorwosidDURMAZ, Rıza/HJH-4918-2023
dc.contributor.authorYang, ZH
dc.contributor.authorDurmaz, R
dc.contributor.authorYang, D
dc.contributor.authorGunal, S
dc.contributor.authorZhang, LX
dc.contributor.authorFoxman, B
dc.contributor.authorSanic, A
dc.date.accessioned2024-08-04T20:15:05Z
dc.date.available2024-08-04T20:15:05Z
dc.date.issued2005
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPrompt detection of drug resistance of Mycobacterium tuberculosis is essential for effective control of tuberculosis (TB). We developed a multiplex allele-specific polymerase chain reaction (MAS-PCR) that detects the most commonly observed isoniazid (M), rifampin (RIF), and ethambutol resistance-associated mutations in a single assay. The usefulness of the newly developed method was evaluated with 174 clinical isolates of M. tuberculosis obtained from Turkey. Distinct PCR banding patterns were observed for different mutation profiles and the correlation between MAS-PCR results and DNA sequencing findings was 99.4%. With culture-based phenotypic drug susceptibility testing as a reference standard, the sensitivity and specificity of the newly developed MAS-PCR assay for drug resistance-related genetic mutation detection were determined to be 81.1% and 97.5% for INH, 93.0% and 98.9% for RIF, and 54.5% and 68.0% for ethambutol. MAS-PCR provides a rapid, potentially more cost-effective, method of detecting multidrug-resistant TB. (c) 2005 Elsevier Inc. All rights reserved.en_US
dc.identifier.doi10.1016/j.diagmicrobio.2005.06.007
dc.identifier.endpage208en_US
dc.identifier.issn0732-8893
dc.identifier.issn1879-0070
dc.identifier.issue3en_US
dc.identifier.pmid16243477en_US
dc.identifier.scopus2-s2.0-27744517828en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage201en_US
dc.identifier.urihttps://doi.org/10.1016/j.diagmicrobio.2005.06.007
dc.identifier.urihttps://hdl.handle.net/11616/94162
dc.identifier.volume53en_US
dc.identifier.wosWOS:000233830600006en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofDiagnostic Microbiology and Infectious Diseaseen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectmultidrug resistanten_US
dc.subjecttuberculosisen_US
dc.subjectmultiplex PCRen_US
dc.titleSimultaneous detection of isoniazid, rifampin, and ethambutol resistance of Mycobacterium tuberculosis by a single multiplex allele-specific polymerase chain reaction (PCR) assayen_US
dc.typeArticleen_US

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