Oxytocin activates calcium signaling in rat sensory neurons through a protein kinase C-dependent mechanism

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dc.authoridOzcan, Mete/0000-0002-5551-4880
dc.authoridKutlu, Selim/0000-0001-9257-4797;
dc.authorwosidOzcan, Sibel/V-9471-2018
dc.authorwosidOzcan, Mete/V-8758-2018
dc.authorwosidAYAR, Ahmet/I-1379-2014
dc.authorwosidKelestimur, Haluk/V-9392-2018
dc.authorwosidKutlu, Selim/AAQ-5501-2021
dc.authorwosidSerhatlioglu, Ihsan/V-9821-2018
dc.contributor.authorAyar, Ahmet
dc.contributor.authorOzcan, Mete
dc.contributor.authorAlcin, Ergul
dc.contributor.authorSerhatlioglu, Ihsan
dc.contributor.authorOzcan, Sibel
dc.contributor.authorKutlu, Selim
dc.contributor.authorKelestimur, Haluk
dc.date.accessioned2024-08-04T20:38:04Z
dc.date.available2024-08-04T20:38:04Z
dc.date.issued2014
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIn addition to its well-known effects on parturition and lactation, oxytocin (OT) plays an important role in modulation of pain and nociceptive transmission. But, the mechanism of this effect is unclear. To address the possible role of OT on pain modulation at the peripheral level, the effects of OT on intracellular calcium levels ([Ca2+](i)) in rat dorsal root ganglion (DRG) neurons were investigated by using an in vitro calcium imaging system. DRG neurons were grown in primary culture following enzymatic and mechanical dissociation of ganglia from 1- or 2-day-old neonatal Wistar rats. Using the fura-2-based calcium imaging technique, the effects of OT on [Ca2+](i) and role of the protein kinase C (PKC)-mediated pathway in OT effect were assessed. OT caused a significant increase in basal levels of [Ca2+](i) after application at the doses of 30 nM (n=34, p<0.01), 100 nM (n=41, p<0.001) and 300 nM (n=46, p<0.001). The stimulatory effect of OT (300 nM) on [Ca2+](i) was persistent in Ca2+-free conditions (n=56, p<0.01). Chelerythrine chloride, a PKC inhibitor, significantly reduced the OT-induced increase in [Ca2+](i) (n=28, p<0.001). We demonstrated that OT activates intracellular calcium signaling in cultured rat primary sensory neurons in a dose-and PKC-dependent mechanism. The finding of the role of OT in peripheral pain modification may serve as a novel target for the development of new pharmacological strategies for the management of pain.en_US
dc.identifier.doi10.1007/s13105-013-0278-z
dc.identifier.endpage48en_US
dc.identifier.issn1138-7548
dc.identifier.issn1877-8755
dc.identifier.issue1en_US
dc.identifier.pmid23912682en_US
dc.identifier.scopus2-s2.0-84896403843en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage43en_US
dc.identifier.urihttps://doi.org/10.1007/s13105-013-0278-z
dc.identifier.urihttps://hdl.handle.net/11616/96363
dc.identifier.volume70en_US
dc.identifier.wosWOS:000332377800005en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal of Physiology and Biochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOxytocinen_US
dc.subjectPainen_US
dc.subjectProtein kinase Cen_US
dc.subjectSensory neuronen_US
dc.subjectCalcium imagingen_US
dc.titleOxytocin activates calcium signaling in rat sensory neurons through a protein kinase C-dependent mechanismen_US
dc.typeArticleen_US

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