Development of nucleic acid based lateral flow assays for SARS-CoV-2 detection

Basit Öğe Kaydı
dc.authoridGULTEKIN, Enes/0000-0002-8545-1868
dc.contributor.authorDerin, Dilek Cam
dc.contributor.authorGultekin, Enes
dc.contributor.authorTaskin, Irmak Icen
dc.contributor.authorYakupogullari, Yusuf
dc.date.accessioned2024-08-04T20:53:14Z
dc.date.available2024-08-04T20:53:14Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractSARS-CoV-2 is still threat for humanity and its detection is crucial. Although real time reverse transcriptase poly-merase chain reaction is the most reliable method for detection of N protein genes, alternative methods for molecular detection are still needed. Thus, lateral flow assay models for 2019-nCoV_ N3 were developed for molecular detection. Briefly, gold nanoparticles were used as label and three sandwich models (1A, 1B, and 1.2) were designed. Prob con-centrations on gold nanoparticles, types of sandwich model and membrane, limit of detection of target gene and buffer efficiency were studied. Model 1B has shown the best results with M170 membrane. Lower limit of detection was achieved by model 1.2 as 5 pM. All parameters have significant role for molecular detection of SARS-CoV-2 by lateral flow assays, and these results will be useful for nucleic acid based lateral flow assays for viral detection or multiple detection of mutated forms in various detection systems.(c) 2022, The Society for Biotechnology, Japan. All rights reserved.en_US
dc.description.sponsorshipInonu University Scientific Research Project (BAP) [TOA-2020-2238]en_US
dc.description.sponsorshipThis work was supported by Inonu University Scientific Research Project (BAP; TOA-2020-2238) .en_US
dc.identifier.doi10.1016/j.jbiosc.2022.11.001
dc.identifier.endpage92en_US
dc.identifier.issn1389-1723
dc.identifier.issn1347-4421
dc.identifier.issue2en_US
dc.identifier.pmid36494247en_US
dc.identifier.scopus2-s2.0-85143969487en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage87en_US
dc.identifier.urihttps://doi.org/10.1016/j.jbiosc.2022.11.001
dc.identifier.urihttps://hdl.handle.net/11616/101058
dc.identifier.volume135en_US
dc.identifier.wosWOS:000934309200001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSoc Bioscience Bioengineering Japanen_US
dc.relation.ispartofJournal of Bioscience and Bioengineeringen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectRapid testen_US
dc.subjectSARS-CoV-2en_US
dc.subjectSandwich assayen_US
dc.subject2019-nCoV_Nen_US
dc.subjectDetectionen_US
dc.titleDevelopment of nucleic acid based lateral flow assays for SARS-CoV-2 detectionen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu