Development of L-asparaginase@hybrid Nanoflowers (ASNase@HNFs) Reactor System with Enhanced Enzymatic Reusability and Stability

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dc.authoridUlu, Ahmet/0000-0002-4447-6233
dc.authoridÖZDEMİR, NALAN/0000-0002-8930-5198
dc.authoridAteş, Burhan/0000-0001-6080-229X
dc.authorwosidNOMA, SAMIR/ABH-1773-2021
dc.authorwosidUlu, Ahmet/L-5180-2016
dc.authorwosidYilmaz, Burcu/HZK-1308-2023
dc.authorwosidÖZDEMİR, NALAN/AAL-7083-2021
dc.authorwosidAteş, Burhan/AAA-3730-2021
dc.contributor.authorNoma, Samir Abbas Ali
dc.contributor.authorYilmaz, Burcu Somturk
dc.contributor.authorUlu, Ahmet
dc.contributor.authorOzdemir, Nalan
dc.contributor.authorAtes, Burhan
dc.date.accessioned2024-08-04T20:48:54Z
dc.date.available2024-08-04T20:48:54Z
dc.date.issued2021
dc.departmentİnönü Üniversitesien_US
dc.description.abstractHybrid nanoflowers materials have recently received great attention in enzyme immobilization applications because of the advantages such as their large surface area, excellent stability, simple, eco-friendly, and cost-effective synthesis. In this study, l-asparaginase which is an important commercial enzyme in the medicine and food industry was selected as a model enzyme. To the best of our knowledge, this study is the first report of designing L-asparaginase@hybrid nanoflowers to enhance its enzymatic performance. L-asparaginase@hybrid nanoflowers were synthesized using ASNase as an organic component and Cu(II) ion as inorganic component. They were characterized by their morphology and chemical point of view by using different techniques. The synthesized L-asparaginase@hybrid nanoflowers exhibited high residual activity at broad pH and high temperature ranges in comparison to free form. Moreover, L-asparaginase@hybrid nanoflowers possessed good reusability and excellent long-time storage stability. Especially, L-asparaginase@hybrid nanoflowers-3 maintained nearly 51 and 75% of its original activity, respectively, after nine consecutive catalytic cycles and storage at 30 degrees C for 4 weeks. The results indicated that these hybrid nanoflowers will be promising carrier matrix for the immobilization of ASNase in biotechnological applications with improved catalytic properties. [GRAPHICS] .en_US
dc.identifier.doi10.1007/s10562-020-03362-1
dc.identifier.endpage1201en_US
dc.identifier.issn1011-372X
dc.identifier.issn1572-879X
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85090246980en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1191en_US
dc.identifier.urihttps://doi.org/10.1007/s10562-020-03362-1
dc.identifier.urihttps://hdl.handle.net/11616/99519
dc.identifier.volume151en_US
dc.identifier.wosWOS:000566028900001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofCatalysis Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHybrid nanoflowersen_US
dc.subjectAsparaginaseen_US
dc.subjectEnzyme immobilizationen_US
dc.subjectImproved stabilityen_US
dc.titleDevelopment of L-asparaginase@hybrid Nanoflowers (ASNase@HNFs) Reactor System with Enhanced Enzymatic Reusability and Stabilityen_US
dc.typeArticleen_US

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