Protective and Therapeutic Effect of Apocynin on Bleomycin-Induced Lung Fibrosis in Rats

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dc.authoridPolat, Alaadin/0000-0002-6920-3856
dc.authoridTaslidere, Elif/0000-0003-1723-2556
dc.authoridVardı, Nigar/0000-0003-0576-1696
dc.authoridParlakpınar, Hakan/0000-0001-9497-3468
dc.authoridParlakpinar, Hakan/0000-0001-9497-3468
dc.authoridErmis, Hilal/0000-0002-1764-9098
dc.authoridPolat, Alaadin/0000-0002-6920-3856
dc.authorwosidPolat, Alaadin/Q-4052-2018
dc.authorwosidTaslidere, Elif/ABI-8046-2020
dc.authorwosidVardı, Nigar/C-9549-2018
dc.authorwosidParlakpınar, Hakan/T-6517-2018
dc.authorwosidParlakpinar, Hakan/V-6637-2019
dc.authorwosidErmis, Hilal/ABI-2900-2020
dc.authorwosidPolat, Alaadin/AAA-7171-2021
dc.contributor.authorKilic, Talat
dc.contributor.authorParlakpinar, Hakan
dc.contributor.authorTaslidere, Elif
dc.contributor.authorYildiz, Sedat
dc.contributor.authorPolat, Alaadin
dc.contributor.authorVardi, Nigar
dc.contributor.authorColak, Cemil
dc.date.accessioned2024-08-04T20:41:09Z
dc.date.available2024-08-04T20:41:09Z
dc.date.issued2015
dc.departmentİnönü Üniversitesien_US
dc.description.abstractWe aimed to investigate the preventive and therapeutic effect of apocynin (APO) on bleomycin (BLC)-induced lung injury in rats. Rats were assigned into groups as follows: control group; APO group, 20 mg/kg APO was given intraperitoneal for 29 days; BLC-1 and BLC-2 groups, a single intratracheal injection of BLC (2.5 mg/kg); APO+BLC-preventive group, 20 mg/kg APO was administered 12 h before the intratracheal BLC injection and continued for 14 days; BLC+APO-treatment group, 20 mg/kg APO was given on the 14th day after the intratracheal BLC injection and continued to sacrifice. The BLC-1 group was sacrificed on the 14th day of BLC administration to validate BLC-induced lung inflammation and fibrosis on the 14th of study initiation. All other groups were sacrificed on the 29th day after BLC administration. The semiquantitative histopathological assessment, tissue levels of malondialdehyde (MDA), superoxide dismutase, catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH), total antioxidant capacity, total oxidant status (TOS), and oxidative stress index (OSI) were measured. An addition to the serum myeloperoxidase (MPO), the cell count and cytokines (IL-1 beta, IL-6, and IL-8) of bronchoalveolar lavage (BAL) fluid were assayed. BLC-provoked histological changes were significantly detected compared to the control group. APO restored these histological damages in different quantity in the treatment and prevention groups. BLC caused a significant decrease in GSH, CAT, and GPX, which were accompanied with significantly the increased MDA, TOS levels, and OSI in the lung tissue concomitant with increased levels of the cellular account and proinflammatory cytokines in the BAL fluid. Otherwise, APO administration, both before and after BLC, reversed all biochemical markers and cytokine as well as histopathological changes induced by BLC. Interestingly, APO treatment reversed MPO activity in serum increased by BLC. In this study, both protective and therapeutic effects of APO against BLC-induced lung fibrosis were demonstrated for the first time.en_US
dc.description.sponsorshipscientific research unit of Inonu University [2013/112]en_US
dc.description.sponsorshipThe authors have no conflict of interest to declare. This work has been supported by the scientific research unit of Inonu University (Project number: 2013/112).en_US
dc.identifier.doi10.1007/s10753-014-0081-1
dc.identifier.endpage1180en_US
dc.identifier.issn0360-3997
dc.identifier.issn1573-2576
dc.identifier.issue3en_US
dc.identifier.pmid25502443en_US
dc.identifier.scopus2-s2.0-84940006566en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1166en_US
dc.identifier.urihttps://doi.org/10.1007/s10753-014-0081-1
dc.identifier.urihttps://hdl.handle.net/11616/96948
dc.identifier.volume38en_US
dc.identifier.wosWOS:000354086100026en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringer/Plenum Publishersen_US
dc.relation.ispartofInflammationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectapocyninen_US
dc.subjectantioxidanten_US
dc.subjectbleomycinen_US
dc.subjectcytokineen_US
dc.subjectpulmonary fibrosisen_US
dc.titleProtective and Therapeutic Effect of Apocynin on Bleomycin-Induced Lung Fibrosis in Ratsen_US
dc.typeArticleen_US

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