A different approach to the quantification of human seminal plasma metabolites using high-resolution NMR spectroscopy

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dc.contributor.authorMumcu, Akin
dc.date.accessioned2024-08-04T20:53:33Z
dc.date.available2024-08-04T20:53:33Z
dc.date.issued2023
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIn this study, a reliable method was established for the absolute quantification of metabolite concentrations in human seminal plasma using ERETIC2, a quantification tool developed by Bruker based on the PULCON principle. The performance of the ERETIC2 was examined using an AVANCE III HD NMR spectrometer (600 MHz) equipped with a triple inverse 1.7 mm TXI probe in terms of some experimental parameters that may affect the accuracy and precision of the quantitative results. Then, the accuracy, precision, and repeatibility of ERETIC2 were determined using L-asparagine solutions at different concentrations. And it was evaluated by comparing it with the classical internal standard (IS) quantification method. The relative standard deviation (RSD) values for ERETIC2 were calculated in the range of 0.55-1.90% and the minimum recovery value was 99.9%, while the RSD values for the IS method were calculated in the range of 0.88-5.83% and recovery value was minimum 91.0%. Besides, the RSD values of the inter-day precisions for the ERETIC2 and IS methods were obtained to be in the range of 1.25 - 3.03% and 0.97 - 3.46%, respectively. Finally, the concentration values of seminal plasma metabolites were determined using different pulse programs with both methods for samples obtained from normozoospermic control and azoospermic patient groups. The results proved that this quantification method developed using NMR spectroscopy is easy to use in complex sample systems such as biological fluids and is a good alternative to the classical internal standard method in terms of accuracy and sensitivity. In addition, the improvement of the spectral resolution and sensitivity with the microcoil probe technology and the possibility of analyzing with minimum sample quantities has contributed positively to the results of this method.en_US
dc.identifier.doi10.1016/j.jpba.2023.115356
dc.identifier.issn0731-7085
dc.identifier.issn1873-264X
dc.identifier.pmid37011551en_US
dc.identifier.scopus2-s2.0-85151239006en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.jpba.2023.115356
dc.identifier.urihttps://hdl.handle.net/11616/101260
dc.identifier.volume229en_US
dc.identifier.wosWOS:000967398200001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectNMR spectroscopyen_US
dc.subjectERETIC2en_US
dc.subjectPULCONen_US
dc.subjectQuantificationen_US
dc.subjectMetabolite concentrationen_US
dc.titleA different approach to the quantification of human seminal plasma metabolites using high-resolution NMR spectroscopyen_US
dc.typeArticleen_US

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