Effect of combined treatment with melatonin and methylprednisolone on neurological recovery after experimental spinal cord injury

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dc.authoridYILMAZ, ÜNSAL/0000-0002-7256-8557
dc.authoridtekiner, ayhan/0000-0002-3835-2568
dc.authoridBatcioglu, Kadir/0000-0001-6663-2287
dc.authoridYologlu, Saim/0000-0002-9619-3462
dc.authorwosidYILMAZ, ÜNSAL/ABG-7549-2021
dc.authorwosidtekiner, ayhan/HIZ-6473-2022
dc.authorwosidBatcioglu, Kadir/Q-2424-2015
dc.authorwosidYologlu, Saim/ABI-8014-2020
dc.contributor.authorCayli, SR
dc.contributor.authorKocak, A
dc.contributor.authorYilmaz, U
dc.contributor.authorTekiner, A
dc.contributor.authorErbil, M
dc.contributor.authorOzturk, C
dc.contributor.authorBatcioglu, K
dc.date.accessioned2024-08-04T20:13:48Z
dc.date.available2024-08-04T20:13:48Z
dc.date.issued2004
dc.departmentİnönü Üniversitesien_US
dc.description.abstractSpinal cord injury (SCI) results in the loss of function below the lesion. Secondary injury following the primary impact includes a number of biochemical and cellular alterations leading to tissue necrosis and cell death. Methylprednisolone (NIP), by reducing edema and protecting the cell membrane against peroxidation, is the only pharmacological agent with a proven clinically beneficial effect on SCI. Melatonin, known as a free radical scavenger, has been shown to have an effect on lipid peroxidation following experimental SCI. The purpose of this study was to examine the effect of MP and melatonin on neurological, ultrastructural, and electrophysiological recovery. Female albino rats weighing 200-250 g were randomized into five groups of 18 rats each and six rats for the control group. Weight-drop trauma was performed for each group and a 30-mg/kg single dose of NIP for rats in group 1, a 10-mg/kg single dose of melatonin for rats in group 2, and MP and melatonin in the same doses for rats in group 3 were administered immediately after trauma. The rats in group 4 were the vehicle group (treated with ethanol) and group 5 was the trauma group. The motor and somatosensory evoked potentials were recorded at the 4th hour, the 24th hour, and on the 10th day of the study for six rats in each group. Posttraumatic neurological recovery was recorded for 10 days using motor function score and inclined plane test. After electrophysiological study the rats were terminated for an analysis of lipid peroxidation level of the injured site of the spinal cord. Electron microscopic studies were performed to determine the effects of melatonin, MP, and the combined treatment with MP and melatonin on axons, neurons, myelin, nucleus, and intracytoplasmic edema. The groups treated with MP, melatonin, and a combination of both had significantly enhanced electrophysiological, biochemical, and neurological recovery and also showed better ultrastructural findings than the trauma and vehicle groups. Although combined treatment was significantly more effective on lipid peroxidation than melatonin or MP treatments alone, at the 10th day, neurobehavioral, electrophysiological, and ultrastructural recovery were at the same level. In conclusion, MP, melatonin, and MP and melatonin combined treatment modalities improved functional recovery at the same level. Future studies involving different doses of melatonin and different dose combinations with MP could promise better results since each drug has a different anti-oxidative mechanism of action.en_US
dc.identifier.doi10.1007/s00586-003-0550-y
dc.identifier.endpage732en_US
dc.identifier.issn0940-6719
dc.identifier.issue8en_US
dc.identifier.pmid15232723en_US
dc.identifier.scopus2-s2.0-15744387138en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage724en_US
dc.identifier.urihttps://doi.org/10.1007/s00586-003-0550-y
dc.identifier.urihttps://hdl.handle.net/11616/93857
dc.identifier.volume13en_US
dc.identifier.wosWOS:000226924200011en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofEuropean Spine Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectspinal cord injuryen_US
dc.subjectmethylprednisoloneen_US
dc.subjectmelatoninen_US
dc.subjectevoked potentialsen_US
dc.subjectlipid peroxidationen_US
dc.titleEffect of combined treatment with melatonin and methylprednisolone on neurological recovery after experimental spinal cord injuryen_US
dc.typeArticleen_US

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