Evaluation of changes in serum lipoprotein and apolipoprotein patterns in cows with ketosis

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dc.authorwosidGüldür, Tayfun/AAA-7088-2021
dc.authorwosidSEZER, KENAN/C-3000-2013
dc.contributor.authorIleri-Buyukoglu, Tulay
dc.contributor.authorSahinduran, Sima
dc.contributor.authorSezer, Kenan
dc.contributor.authorGuldur, Tayfun
dc.date.accessioned2024-08-04T20:31:14Z
dc.date.available2024-08-04T20:31:14Z
dc.date.issued2009
dc.departmentİnönü Üniversitesien_US
dc.description.abstractObjective-To detect changes in serum lipoprotein and apolipoprotein profiles via precipitation and electrophoresis in ketotic cows and in those cows treated with different methods. Animals-21 cows with clinical and subclinical ketosis, 7 healthy cows in the early lactation period, and 7 healthy cows in the nonlactation period. Procedures-Ketotic cows were allocated into 3 groups; the first group was treated with dextrose and dexamethasone, the second group with dextrose and prednisolone, and the third group with dextrose and insulin. The beta and alpha lipoproteins were precipitated with dextran sulfate-magnesium chloride in ketotic cows after treatment and healthy cows in the nonlactation and lactation periods. The serum samples, precipitates, and supernatants were examined via agarose gel electrophoresis for detection of alterations in serum lipoproteins. Subsequently, alterations in serum apolipoproteins were detected via SDS-PAGE of precipitates. Results-Compared with serum beta and alpha lipoprotein concentrations in healthy cows during nonlactation, those in cows during lactation were higher; however, those in cows with ketosis were lower. The SDS-PAGE analysis of serum beta lipoproteins revealed that apolipoprotein E (approx 36 and 40 kDa) decreased in ketotic cows, in comparison with healthy cows in the nonlactation and lactation periods, but increased after treatment. Decreases in apolipoprotein B (approx 222 kDa), apolipoprotein A-I(19 and 24 kDa), apolipoprotein A-IV (55 kDa), apolipoprotein C-III (8.8 and 10.2 kDa), and albumin (66 kDa) concentrations were detected in ketotic cows, in comparison with the healthy cows in the lactation period. Conclusions and Clinical Relevance-Serum lipoprotein and apolipoproteins may routinely be determined via precipitation and electrophoresis in the diagnosis and treatment of ketosis. (Am J Vet Res 2009;70:563-570)en_US
dc.identifier.doi10.2460/ajvr.70.5.563
dc.identifier.endpage570en_US
dc.identifier.issn0002-9645
dc.identifier.issue5en_US
dc.identifier.pmid19405893en_US
dc.identifier.scopus2-s2.0-65749118742en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage563en_US
dc.identifier.urihttps://doi.org/10.2460/ajvr.70.5.563
dc.identifier.urihttps://hdl.handle.net/11616/94802
dc.identifier.volume70en_US
dc.identifier.wosWOS:000265550500001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Veterinary Medical Assocen_US
dc.relation.ispartofAmerican Journal of Veterinary Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectA-I Concentrationsen_US
dc.subjectPeriparturient Dairy-Cowsen_US
dc.subjectFatty Liveren_US
dc.subjectPlasma-Lipoproteinsen_US
dc.subjectC-Iiien_US
dc.subjectLactating Cowen_US
dc.subjectBovine Serumen_US
dc.subjectPrecipitation Methodsen_US
dc.subjectLeft Displacementen_US
dc.subjectDextran Sulfateen_US
dc.titleEvaluation of changes in serum lipoprotein and apolipoprotein patterns in cows with ketosisen_US
dc.typeArticleen_US

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