Real-time cell analysis of the cytotoxicity of orthodontic brackets on gingival fibroblasts

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dc.authoridHakkı, Sema S/0000-0001-8665-6235
dc.authorwosidHakkı, Sema S/Y-2878-2018
dc.contributor.authorToy, Ebubekir
dc.contributor.authorMalkoc, Siddik
dc.contributor.authorCorekci, Bayram
dc.contributor.authorBozkurt, Buket S.
dc.contributor.authorHakki, Sema S.
dc.date.accessioned2024-08-04T20:40:08Z
dc.date.available2024-08-04T20:40:08Z
dc.date.issued2014
dc.departmentİnönü Üniversitesien_US
dc.description.abstractPurpose: The aim of this study was to evaluate the cytotoxic effects of 6 different orthodontic bracket types on human gingival fibroblasts (HGFs) using the xCELLigence system. Methods: The orthodontic brackets used in this study were gold-plated steel (Apollo Gold), titanium (Rematitan), stainless steel (Equilibrium 2), lucid ice (Inspire ICE), metal-reinforced ceramic (Clarity) and composite (OrthoFlex). Gingival fibroblasts were isolated from human gingival connective tissue of systemically healthy individuals. Tested brackets were incubated in DMEM culture medium for 72 hours according to ISO 10993-5 standards. Gingival fibroblasts were maintained with Dulbecco modified Eagle medium containing 10% fetal bovine serum. The xCELLigence system was used to evaluate cell survival. The statistical analysis used was ANOVA and Tukey-Kramer multiple comparison tests. Results: When the data were evaluated in the 30th hour, Apollo Gold showed significant decreases in cell index (P<0.001). It also showed statistically significant decreases (P<0.001) in the 65th hour, but Clarity and Inspire ICE showed significant increases in cell indices (P<0.001, P<0.01). In the 114th hour, Clarity and Equilibrium 2 showed statistically significant increases in cell indices (P<0.001). Inspire ICE and Rematitan demonstrated significant increases (P<0.05). There were significant decreases in cell index of Apollo Gold (P<0.001). Conclusions: The tested brackets are suitable for clinical application, but further studies using different test methods are needed for gold-plated brackets.en_US
dc.identifier.doi10.5301/JABFM.5000165
dc.identifier.endpage255en_US
dc.identifier.issn2280-8000
dc.identifier.issue3en_US
dc.identifier.pmid24425375en_US
dc.identifier.scopus2-s2.0-84925296534en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage248en_US
dc.identifier.urihttps://doi.org/10.5301/JABFM.5000165
dc.identifier.urihttps://hdl.handle.net/11616/96716
dc.identifier.volume12en_US
dc.identifier.wosWOS:000347554200017en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSage Publications Ltden_US
dc.relation.ispartofJournal of Applied Biomaterials & Functional Materialsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCytotoxicityen_US
dc.subjectHuman gingival fibroblastsen_US
dc.subjectOrthodontic bracketsen_US
dc.subjectxCELLence systemen_US
dc.titleReal-time cell analysis of the cytotoxicity of orthodontic brackets on gingival fibroblastsen_US
dc.typeArticleen_US

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