Chloro-Modified Magnetic Fe3O4@MCM-41 Core-Shell Nanoparticles for L-Asparaginase Immobilization with Improved Catalytic Activity, Reusability, and Storage Stability

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dc.authoridUlu, Ahmet/0000-0002-4447-6233
dc.authoridAteş, Burhan/0000-0001-6080-229X
dc.authoridKoytepe, Suleyman/0000-0002-4788-278X
dc.authorwosidUlu, Ahmet/L-5180-2016
dc.authorwosidAteş, Burhan/AAA-3730-2021
dc.authorwosidKöytepe, Süleyman/AAA-4168-2021
dc.authorwosidNOMA, SAMIR/ABH-1773-2021
dc.contributor.authorUlu, Ahmet
dc.contributor.authorNoma, Samir Abbas Ali
dc.contributor.authorKoytepe, Suleyman
dc.contributor.authorAtes, Burhan
dc.date.accessioned2024-08-04T20:45:22Z
dc.date.available2024-08-04T20:45:22Z
dc.date.issued2019
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThis paper describes a new support that permits to efficient immobilization of L-asparaginase (L-ASNase). For this purpose, Fe3O4 magnetic nanoparticles were synthesized and coated by MCM-41. 3-chloropropyltrimethoxysilane (CPTMS) was used as a surface modifying agent for covalent immobilization of L-ASNase on the magnetic nanoparticles. The chemical structure; thermal, morphological, and magnetic properties; chemical composition; and zeta potential value of Fe3O4@MCM-41-Cl were characterized by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential thermal analysis (DTA), differential scanning calorimetry (DSC), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), energy dispersive X-ray (EDX), X-ray diffraction patterns (XRD), and zeta-potential measurement. The immobilization efficiency onto Fe3O4@MCM-41-Cl was detected as 63%. The reusability, storage, pH, and thermal stabilities of the immobilized L-ASNase were investigated and compared to that of soluble one. The immobilized enzyme maintained 42.2% of its original activity after 18cycles of reuse. Furthermore, it was more stable towards pH and temperature compared with soluble enzyme. The Michaelis-Menten kinetic properties of immobilized L-ASNase showed a lower V-max and a similar K-m compared to soluble L-ASNase. Immobilized enzyme had around 47 and 32.5% residual activity upon storage a period of 28days at 4 and 25 degrees C, respectively. In conclusion, the Fe3O4@MCM-41-Cl@L-ASNase core-shell nanoparticles could successfully be used in industrial and medical applications.en_US
dc.description.sponsorshipInonu University [FDK-2017-751]en_US
dc.description.sponsorshipThis study was partially supported by from Inonu University (FDK-2017-751).en_US
dc.identifier.doi10.1007/s12010-018-2853-9
dc.identifier.endpage956en_US
dc.identifier.issn0273-2289
dc.identifier.issn1559-0291
dc.identifier.issue3en_US
dc.identifier.pmid30101367en_US
dc.identifier.scopus2-s2.0-85052058208en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage938en_US
dc.identifier.urihttps://doi.org/10.1007/s12010-018-2853-9
dc.identifier.urihttps://hdl.handle.net/11616/98437
dc.identifier.volume187en_US
dc.identifier.wosWOS:000463838900018en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofApplied Biochemistry and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFe3O4@MCM-41en_US
dc.subjectCore-shell magnetic particlesen_US
dc.subjectChloro groupen_US
dc.subjectL-asparaginaseen_US
dc.subjectEnzyme immobilizationen_US
dc.titleChloro-Modified Magnetic Fe3O4@MCM-41 Core-Shell Nanoparticles for L-Asparaginase Immobilization with Improved Catalytic Activity, Reusability, and Storage Stabilityen_US
dc.typeArticleen_US

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