Comparison of stereochemical structures of cholesterol from different sources by HPLC

Basit Öğe Kaydı
dc.authoridKarakurt, Arzu/0000-0003-2209-0871
dc.authoridSatilmis, Basri/0000-0002-2538-5774
dc.authoridSatilmis, Basri/0000-0002-2538-5774
dc.authoridBuyuktuncel, Ebru/0000-0001-7269-6594
dc.authorwosidKarakurt, Arzu/ABH-9340-2020
dc.authorwosidSatilmis, Basri/JBR-9078-2023
dc.authorwosidSatilmis, Basri/C-5176-2019
dc.authorwosidBuyuktuncel, Saliha Ebru/ABH-9343-2020
dc.authorwosidGüldür, Tayfun/AAA-7088-2021
dc.contributor.authorSatilmis, Basri
dc.contributor.authorGuldur, Tayfun
dc.contributor.authorKarakurt, Arzu
dc.contributor.authorBuyuktuncel, Ebru
dc.contributor.authorErtan, Mevlut
dc.date.accessioned2024-08-04T20:37:38Z
dc.date.available2024-08-04T20:37:38Z
dc.date.issued2012
dc.departmentİnönü Üniversitesien_US
dc.description.abstractIt is known that only one stereoisomeric form, nat-cholesterol, naturally occurs. Nat-cholesterol and its enantiomer, ent-cholesterol, sometimes show enantiospecific interactions with biological molecules. If cholesterol is naturally found only one form, then the question of why does cholesterol show an enantiomeric selectivity? arises. For this purpose, stereoisomer analysis of cholesterol obtained from porcine liver and wool wax were carried out with three different high performance liquid chromatography (HPLC) systems including reversed-phase, reversed-phase with different cyclodextrins as a mobile phase modifier, and chiral. Results from HPLC analysis of both cholesterol samples by permethylated gamma-cyclodextrin and amylose tris-(3,5-dimethylphenylcarbamate) chiral columns showed that there was no stereoisomer of cholesterol present. However reversed-phase HPLC analysis of cholesterol samples from porcine liver carried out with various cyclodextrins as mobile phase modifiers presented a peak which was not observed in the analysis of cholesterol samples from wool wax. On the other hand, different storage conditions of cholesterol samples and addition of cyclodextrins as mobile phase modifiers produced almost identical alterations in chromatograms of fresh samples by reversed-phase HPLC. This could be attributed to catalytic properties of cyclodextrins. Cyclodextrins may not be suitable as a mobile phase modifier in the stereoisomer analysis of cholesterol with high performance liquid chromatography.en_US
dc.identifier.doi10.12991/201216399
dc.identifier.endpage205en_US
dc.identifier.issn1309-0801
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-84878776595en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage201en_US
dc.identifier.urihttps://doi.org/10.12991/201216399
dc.identifier.urihttps://hdl.handle.net/11616/96088
dc.identifier.volume16en_US
dc.identifier.wosWOS:000216821900006en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMarmara Univ, Fac Pharmacyen_US
dc.relation.ispartofMarmara Pharmaceutical Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCholesterolen_US
dc.subjectstereoisomeren_US
dc.subjectchromatographyen_US
dc.subjectchiralen_US
dc.subjectcyclodextrinen_US
dc.titleComparison of stereochemical structures of cholesterol from different sources by HPLCen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu