Resveratrol and quercetin-induced apoptosis of human 232B4 chronic lymphocytic leukemia cells by activation of caspase-3 and cell cycle arrest

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dc.authoridBaran, Yusuf/0000-0002-1056-4673
dc.authoridadan, aysun/0000-0002-3747-8580
dc.authorwosidApohan, Elif/AAA-5142-2021
dc.contributor.authorGokbulut, Aysun Adan
dc.contributor.authorApohan, Elif
dc.contributor.authorBaran, Yusuf
dc.date.accessioned2024-08-04T20:37:35Z
dc.date.available2024-08-04T20:37:35Z
dc.date.issued2013
dc.departmentİnönü Üniversitesien_US
dc.description.abstractChronic lymphocytic leukemia (CLL), defined by accumulation of pathogenic B cells, has a very complex biology due to various factors such as inherited, host, and enviromental factors. Recently, finding new therapeutic agents or development of novel treatment strategies have been paid attention. Resveratrol and quercetin, important phytoalexins found in many plants, have been reported to have cytotoxic effects on various types of cancer. In this study, we examined cytotoxic, cytostatic, and apoptotic effects of these two important phenolic compounds on 232B4 human CLL cells. Cytotoxic effects of resveratrol and quercetin were determined by MTT cell proliferation assay. Changes in caspase-3 enzyme activity were measured using caspase-3 colorimetric assay. Annexin V-FITC/PI double staining was performed to measure apoptotic cell population. Effects of resveratrol and quercetin on cell cycle profiles of CLL cells were investigated by flow cytometry. Treatment of CLL cells with resveratrol and quercetin caused dose dependent inhibition of cell proliferation and increased apoptotic cell population through induction of caspase-3 activity. Cell cycle analysis displayed cell cycle arrest mainly in G0/G1 for both polyphenols. Our data, in total, showed for the first time that resveratrol and quercetin might block CLL growth through inducing apoptosis and cell cycle arrest.en_US
dc.description.sponsorshipTurkish Academy of Sciences Outstanding Young Investigator Programmeen_US
dc.description.sponsorshipWe thank Biotechnology and Bioengineering Center staff of Izmir Institute of Technology for their help and technical support. This study was supported by the Turkish Academy of Sciences Outstanding Young Investigator Programme.en_US
dc.identifier.doi10.1179/1607845412Y.0000000042
dc.identifier.endpage150en_US
dc.identifier.issn1024-5332
dc.identifier.issn1607-8454
dc.identifier.issue3en_US
dc.identifier.pmid23432965en_US
dc.identifier.scopus2-s2.0-84877912111en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage144en_US
dc.identifier.urihttps://doi.org/10.1179/1607845412Y.0000000042
dc.identifier.urihttps://hdl.handle.net/11616/96061
dc.identifier.volume18en_US
dc.identifier.wosWOS:000319330900004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofHematologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectChronic lymphocytic leukemiaen_US
dc.subjectResveratrolen_US
dc.subjectQuercetinen_US
dc.subjectApoptosisen_US
dc.titleResveratrol and quercetin-induced apoptosis of human 232B4 chronic lymphocytic leukemia cells by activation of caspase-3 and cell cycle arresten_US
dc.typeArticleen_US

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