Design of laccase-metal-organic framework hybrid constructs for biocatalytic removal of textile dyes

Basit Öğe Kaydı
dc.authoridUlu, Ahmet/0000-0002-4447-6233
dc.authoridAteş, Burhan/0000-0001-6080-229X
dc.authoridYesilada, Ozfer/0000-0003-0038-6575
dc.authoridBoran, Filiz/0000-0002-8801-7987
dc.authorwosidUlu, Ahmet/L-5180-2016
dc.authorwosidAteş, Burhan/AAA-3730-2021
dc.authorwosidYesilada, Ozfer/ABI-1335-2020
dc.contributor.authorBirhanli, Emre
dc.contributor.authorNoma, Samir Abbas Ali
dc.contributor.authorBoran, Filiz
dc.contributor.authorUlu, Ahmet
dc.contributor.authorYesilada, Ozfer
dc.contributor.authorAtes, Burhan
dc.date.accessioned2024-08-04T20:50:57Z
dc.date.available2024-08-04T20:50:57Z
dc.date.issued2022
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThis study aims to present a simple and effective carrier matrix to immobilize laccase as opposed to complex and tedious immobilization processes and also to use it in the removal of textile dyes. For this purpose, Cobalt (Co) and Copper (Cu) based metal-organic frameworks (MOFs) were prepared and laccase was immobilized on two different MOFs via encapsulation. The characterization outcomes showed that laccase was well immobilized into MOF supports. Optimum pH and temperature were found for Lac/Co-MOF (pH 4.5 at 50 degrees C) and Lac/Cu-MOF (pH 5.0 at 50 degrees C). The Km (0.03 mM) and Vmax (97.4 mu mol/min) values of Lac/Cu-MOF were lower than those of Lac/Co-MOF (Km = 0.13 mM, Vmax = 230.7 mu mol/min). The immobilized laccases showed good reusability as well as improved resistance to temperature denaturation and high storage stability. For instance, the Lac/Co-MOF and Lac/Cu-MOF retained more than 58% activity after 4 weeks of storage at room temperature. Meanwhile, Lac/Co-MOF and Lac/Cu-MOF maintained 56.5% and 55.8% of their initial activity, respectively, after 12 reuse cycles. Moreover, thermal deactivation kinetic studies of immobilized laccases displayed lower k value, higher t1/2, and enhancement of thermodynamic parameters, which means better thermostability. Finally, the decolorization activities for the Lac/Co-MOF were 78% and 61% at the 5th cycle for Reactive Blue 171 anden_US
dc.identifier.doi10.1016/j.chemosphere.2021.133382
dc.identifier.issn0045-6535
dc.identifier.issn1879-1298
dc.identifier.pmid34954196en_US
dc.identifier.scopus2-s2.0-85121769416en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.chemosphere.2021.133382
dc.identifier.urihttps://hdl.handle.net/11616/100381
dc.identifier.volume292en_US
dc.identifier.wosWOS:000758315800007en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofChemosphereen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLaccaseen_US
dc.subjectEnzyme immobilizationen_US
dc.subjectMetal-organic frameworksen_US
dc.subjectTextile dyeen_US
dc.subjectDecolorizationen_US
dc.subjectWastewater remediationen_US
dc.titleDesign of laccase-metal-organic framework hybrid constructs for biocatalytic removal of textile dyesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu