Analysis of Swimming, Swarming and Twitching Movements of Pseudomonas aeruginosa and its Recombinant Strain
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Tarih
2021
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info:eu-repo/semantics/openAccess
Özet
Pseudomonas aeruginosa has several flagella at a single pole. Flagella helps bacteria to settle in solid food. They also perform the swimming movement, which is the movement in the liquid medium, with flagella. In the study, P. aeruginosa ATCC 10145 (NRRL B-771) and recombinant P. aeruginosa integrated into the chromosome of this bacterium by homologous recombination as a single copy vgb was used. While not observed, it was observed that its recombinant strain also had a pronounced movement of about 2-9 mm. While wild bacteria showed a twitching motion of about 0.2 mm, recombinant bacteria showed a tremor movement of 0.4-0.5 mm. While a small amount of significant swimming movement of approximately 0.2 mm was observed in wild bacteria, it was observed that there was a much more pronounced movement of 1.1-2.2 mm in recombinant bacteria. When the sliding motion was examined at the end of the 24-hour period, it was concluded that there was an obvious movement up to 30 times compared to the wild type of the recombinant strain. When the bacteria were examined in terms of the tremor action, it was found that the recombinant strain showed about 2.5 times more shivering movement than the wild type at the end of the 24-hour period. When the bacteria were observed in terms of the swimming test, it was observed that the recombinant strain made approximately 10 times more swimming movements than the wild type at the end of the 24-hour period.
Açıklama
Anahtar Kelimeler
Kaynak
Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi
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Cilt
14
Sayı
2
