Assessment of the Genetic Diversity of Mycobacterium tuberculosis esxA, esxH, and fbpB Genes among Clinical Isolates and Its Implication for the Future Immunization by New Tuberculosis Subunit Vaccines Ag85B-ESAT-6 and Ag85B-TB10.4

Basit Öğe Kaydı
dc.authoridDURMAZ, RIZA/0000-0001-6561-778X
dc.authorwosidZhang, Li/GWM-7501-2022
dc.authorwosidDURMAZ, Rıza/HJH-4918-2023
dc.contributor.authorDavila, Jose
dc.contributor.authorZhang, Lixin
dc.contributor.authorMarrs, Carl F.
dc.contributor.authorDurmaz, Riza
dc.contributor.authorYang, Zhenhua
dc.date.accessioned2024-08-04T20:32:31Z
dc.date.available2024-08-04T20:32:31Z
dc.date.issued2010
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThe effort to develop a tuberculosis (TB) vaccine more effective than the widely used Bacille Calmette-Guerin (BCG) has led to the development of two novel fusion protein subunit vaccines: Ag85B-ESAT-6 and Ag85B-TB10.4. Studies of these vaccines in animal models have revealed their ability to generate protective immune responses. Yet, previous work on TB fusion subunit vaccine candidate, Mtb72f, has suggested that genetic diversity among M. tuberculosis strains may compromise vaccine efficacy. In this study, we sequenced the esxA, esxH, and fbpB genes of M. tuberculosis encoding ESAT-6, TB10.4, and Ag85B proteins, respectively, in a sample of 88 clinical isolates representing 57 strains from Ark, USA, and 31 strains from Turkey, to assess the genetic diversity of the two vaccine candidates. We found no DNA polymorphism in esxA and esxH genes in the study sample and only one synonymous single nucleotide change (C to A) in fbpB gene among 39 (44.3%) of the 88 strains sequenced. These data suggest that it is unlikely that the efficacy of Ag85B-ESAT-6 and Ag85B-TB10.4 vaccines will be affected by the genetic diversity of M. tuberculosis population. Future studies should include a broader pool of M. tuberculosis strains to validate the current conclusion.en_US
dc.description.sponsorshipNational Institutes of Health [NIH-R01-AI151975]; Office of the Vice President for Research of the University of Michiganen_US
dc.description.sponsorshipThis study was supported by Grant NIH-R01-AI151975 from the National Institutes of Health and the Research Fund of the Office of the Vice President for Research of the University of Michigan. The Arkansas isolates and the genotyping data of the study isolated used in this study were kindly provided by Dr. Joseph H. Bates at the Arkansas Department of Health and Dr. Donald M. Cave at the University of Arkansas for Medical Sciences.en_US
dc.identifier.doi10.1155/2010/208371
dc.identifier.issn1110-7243
dc.identifier.issn1110-7251
dc.identifier.pmid20617139en_US
dc.identifier.scopus2-s2.0-77955392735en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.urihttps://doi.org/10.1155/2010/208371
dc.identifier.urihttps://hdl.handle.net/11616/95099
dc.identifier.wosWOS:000280878100001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherHindawi Ltden_US
dc.relation.ispartofJournal of Biomedicine and Biotechnologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAntigen 85ben_US
dc.subjectEsat-6en_US
dc.subjectFusionen_US
dc.subjectPolymorphismen_US
dc.subjectProtectionen_US
dc.subjectEfficacyen_US
dc.subjectComplexen_US
dc.titleAssessment of the Genetic Diversity of Mycobacterium tuberculosis esxA, esxH, and fbpB Genes among Clinical Isolates and Its Implication for the Future Immunization by New Tuberculosis Subunit Vaccines Ag85B-ESAT-6 and Ag85B-TB10.4en_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu