A computational structural analysis of functional attributes of hypodermin A and B proteins: A way forward for vaccine development

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dc.authoridAfzal, Muhammad Sohail/0000-0001-9807-5710
dc.authoridAfzal, Muhammad Inam/0000-0002-7338-6237
dc.authoridUmer, Muhammad/0009-0001-8751-6100
dc.authoridAhmed, Haroon/0000-0002-0382-3569
dc.authoridSimsek, Sami/0000-0002-3567-326X
dc.authoridKhalid, Rana Rehan/0000-0003-4682-4032
dc.authorwosidMaryam, Arooma/GRJ-6289-2022
dc.authorwosidSiddiqi, Abdul Rauf/P-1625-2015
dc.authorwosidAfzal, Muhammad Sohail/J-4930-2017
dc.authorwosidAfzal, Muhammad Inam/AAD-5165-2019
dc.authorwosidUmer, Muhammad/KHU-2339-2024
dc.authorwosidAhmed, Haroon/S-4085-2019
dc.authorwosidSimsek, Sami/V-9771-2018
dc.contributor.authorArif, Irum
dc.contributor.authorSiddiqi, Abdul Rauf
dc.contributor.authorAhmed, Haroon
dc.contributor.authorAfzal, Muhammad Inam
dc.contributor.authorUmer, Muhammad
dc.contributor.authorMaryam, Arooma
dc.contributor.authorKhalid, Rana Rehan
dc.date.accessioned2024-08-04T20:45:30Z
dc.date.available2024-08-04T20:45:30Z
dc.date.issued2018
dc.departmentİnönü Üniversitesien_US
dc.description.abstractHypodermosis is a parasitic disease of cattle. The pathogenicity of the disease is attributed to Hypodermin proteins (Hypodermin A, Hypodermin B and Hypodermin C). Studies suggest that Hypodermin proteins may be defined as Serine proteases and collagenases. The structure of both proteases Hypodermin A and Hypodermin B were modeled using the Swiss-model server followed by its validation using Procheck, Errat and Verify-3D. Afterwards, both Hypodermin A and Hypodermin B were docked against collagen in order to study its interaction with respective Hypodermin proteins. The structure of both Hypodermin A and Hypodermin B showed more bent towards hydrophobic nature as more beta sheets were present in them. Both structures were also superimposed to check out similarities and differences present between them. Serine, Aspartic acid, Histidine, Glutamic acid and Lysine are found as interacting residues that are involved in hydrogen bonding with collagen. The interactions are found in the active domain region of Hypodermin proteins. The interacting residues were present in the active region of the hypodermin proteins thus validating the docking studies. This study may help in the drug development against hypodermosis with least side effects.en_US
dc.description.sponsorshipTUBITAK (2216- Research Fellowship Program for International Researchers)en_US
dc.description.sponsorshipWe are very thankful to TUBITAK (2216- Research Fellowship Program for International Researchers) to provide an opportunity and funding to work for Dr. Haroon Ahmed. IA and ARS contributed equally to this work.en_US
dc.identifier.endpage2451en_US
dc.identifier.issn1011-601X
dc.identifier.issue6en_US
dc.identifier.pmid30473516en_US
dc.identifier.scopus2-s2.0-85055163073en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage2443en_US
dc.identifier.urihttps://hdl.handle.net/11616/98502
dc.identifier.volume31en_US
dc.identifier.wosWOS:000455072400018en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherUniv Karachien_US
dc.relation.ispartofPakistan Journal of Pharmaceutical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHypodermosisen_US
dc.subjecthypodermin Aen_US
dc.subjecthypodermin Ben_US
dc.subjectcollagenen_US
dc.subjectmodelingen_US
dc.subjectdockingen_US
dc.titleA computational structural analysis of functional attributes of hypodermin A and B proteins: A way forward for vaccine developmenten_US
dc.typeArticleen_US

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