Identification of bacterial species in milk by MALDI-TOF and assessment of some oxidant-antioxidant parameters in blood and milk from cows with different health status of the udder

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dc.authoridZigo, František/0000-0002-2791-166X
dc.authoridYılmaz, Seval/0000-0002-2040-9247
dc.authoridÇAMBAY, Zafer/0000-0002-1170-7525
dc.authoridÖzçelik, Mehtap/0000-0003-2362-4935
dc.authoridOtlu, Baris/0000-0002-6220-0521
dc.authorwosidZigo, František/AEO-3938-2022
dc.authorwosidYılmaz, Seval/V-9042-2018
dc.authorwosidÇAMBAY, Zafer/V-8870-2018
dc.authorwosidÖzçelik, Mehtap/V-8816-2018
dc.contributor.authorOzbey, G.
dc.contributor.authorCambay, Z.
dc.contributor.authorYilmaz, S.
dc.contributor.authorAytekin, O.
dc.contributor.authorZigo, F.
dc.contributor.authorOzcelik, M.
dc.contributor.authorOtlu, B.
dc.date.accessioned2024-08-04T20:52:13Z
dc.date.available2024-08-04T20:52:13Z
dc.date.issued2022
dc.departmentİnönü Üniversitesien_US
dc.description.abstractThis study aimed to identify bacterial pathogens in milk samples from dairy cows with sub -clinical and clinical mastitis as well as to assess the concentrations of oxidant-antioxidant param-eters [malondialdehyde (MDA), reduced glutathione (GSH), and total GSH levels] in both blood and milk samples. From a total of 200 dairy cows in 8 farms, 800 quarter milk samples obtained from each udder were tested in the laboratory for the presence of udder pathogens. Cultivated bacteria causing intramammary infection from milk samples were identified by Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF). In addition, from tested animals 60 cows were selected includ??ng 20 healthy cows that were CMT negative, 20 cows with subclini-cal mastitis (SM), and 20 cows with clinical mastitis (CM) for detection of MDA, GSH, and total GSH levels in blood and milk samples. Three hundred and eighty (47.5%; 380/800), 300 (37.5%; 300/800), and 120 (15%; 120/800) of milk samples, respectively were CMT positive or SM and CM, and those positives were cows from different farms. We observed that 87.4% (332/380), 25.3% (76/300), and 34.2% (41/120) of cows with CMT positive, CMT negative, and CM had bacterial growth. The most predominantly identified bacteria were Staphylococcus chromogenes (18.7%) obtained mainly from SM and Staphylococcus aureus (16.7%) as the most frequent cau-se of CM. According to our results, dairy cows with CM had the highest MDA levels, the lowest GSH, and total GSH levels in both blood and milk samples however, high MDA levels and low GSH levels in milk samples with SM were observed. Based on our results, lipid oxidant MDA and antioxidant GSH could be excellent biomarkers of cow???s milk for developing inflammation of the mammary gland. In addition, there was no link between nutrition and MDA and GSH levels.en_US
dc.description.sponsorshipDepartment of Medical Microbiology, Faculty of Medicine, Inonu University, Malatya, Turkey; Slovak grant KEGA [006UVLF-4-2020.]en_US
dc.description.sponsorshipThis research was funded by Professor Baris OTLU from Department of Medical Microbiology, Faculty of Medicine, Inonu University, Malatya, Turkey and Slovak grant KEGA no. 006UVLF-4-2020.en_US
dc.identifier.doi10.24425/pjvs.2022.141811
dc.identifier.endpage277en_US
dc.identifier.issn1505-1773
dc.identifier.issn2300-2557
dc.identifier.issue2en_US
dc.identifier.pmid35861970en_US
dc.identifier.scopus2-s2.0-85134854951en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage269en_US
dc.identifier.urihttps://doi.org/10.24425/pjvs.2022.141811
dc.identifier.urihttps://hdl.handle.net/11616/100806
dc.identifier.volume25en_US
dc.identifier.wosWOS:000827899600009en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPolska Akad Nauk, Polish Acad Sciences, Univ Warmia & Mazury Olsztynen_US
dc.relation.ispartofPolish Journal of Veterinary Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectdairy cowsen_US
dc.subjectCMTen_US
dc.subjectmastitisen_US
dc.subjectmalondialdehydeen_US
dc.subjectglutathioneen_US
dc.subjectMALDI-TOFen_US
dc.titleIdentification of bacterial species in milk by MALDI-TOF and assessment of some oxidant-antioxidant parameters in blood and milk from cows with different health status of the udderen_US
dc.typeArticleen_US

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