Visualization and characterization ofEnterococcus faecalisbiofilm structure in bovine dentin using 2D and 3D microscopic techniques

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dc.authoridKeskin, Cangul/0000-0001-8990-4847
dc.authoridKeleş, Ali/0000-0003-2835-767X
dc.authoridGül, Mehmet/0000-0002-1374-0783
dc.authoridSahin, Fikrettin/0000-0003-1503-5567
dc.authorwosidKeskin, Cangul/ACA-8702-2022
dc.authorwosidKeleş, Ali/J-5897-2015
dc.authorwosidYAKUPOGULLARI, YUSUF/F-3966-2011
dc.authorwosidGül, Mehmet/ABI-6336-2020
dc.authorwosidErtan, Asli/AAE-6201-2021
dc.contributor.authorKeles, Ali
dc.contributor.authorKeskin, Cangul
dc.contributor.authorKalkan, Melis
dc.contributor.authorYakupogullari, Yusuf
dc.contributor.authorGul, Mehmet
dc.contributor.authorAydemir, Hikmet
dc.contributor.authorSahin, Fikrettin
dc.date.accessioned2024-08-04T20:48:55Z
dc.date.available2024-08-04T20:48:55Z
dc.date.issued2021
dc.departmentİnönü Üniversitesien_US
dc.description.abstractBacterial biofilms are related to various dental and periodontal infectious diseases, and the characterization of this biological structure with micro-computed tomography (micro-CT) may offer valuable information for clinical and research applications. In this study, we aimed to develop a model to visualize three-dimensionally the biofilm structure on dentin using micro-CT. Dentin blocks were prepared and incubated in tryptic soy broth withEnterococcus faecalis(ATCC 29212). The control group did not receive any staining procedure, while groups 1 and 2 were stained with 100% and 50% barium sulfate, respectively. Transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM) were used to detect biofilm formation, barium sulfate penetration, and microbial cell density in the biofilm. Micro-computed tomography (micro-CT) (SkyScan 1172, Bruker Co., Belgium) was used to visualize biofilm formation on the dentin blocks. Biofilm thicknesses were measured from 10 different locations on the specimen surfaces, using CTAn v.1.14.4 software. Obtained data were statistically analyzed using Kruskal-Wallis and Dunn's tests. TEM photomicrographs showed that barium sulfate could penetrate the biofilm structure. CLSM analysis showed that viable and total cell densities were similar between the control and barium sulfate-treated groups (P > 0.05), indicating barium sulfate had no significant influence on cell density. In barium sulfate-treated blocks, biofilm could be discriminated from the dentin, and its thickness could be measured with micro-CT. This study showed that bacterial biofilm on dentin could be characterized by micro-CT after barium sulfate staining without causing any significant side effect on viable and total cell densities.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey-TUBITAK [114S511]en_US
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council of Turkey-TUBITAK (Grant no. 114S511).en_US
dc.identifier.doi10.1007/s00203-020-02031-6
dc.identifier.endpage277en_US
dc.identifier.issn0302-8933
dc.identifier.issn1432-072X
dc.identifier.issue1en_US
dc.identifier.pmid32918096en_US
dc.identifier.scopus2-s2.0-85090772452en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage269en_US
dc.identifier.urihttps://doi.org/10.1007/s00203-020-02031-6
dc.identifier.urihttps://hdl.handle.net/11616/99533
dc.identifier.volume203en_US
dc.identifier.wosWOS:000568483400003en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofArchives of Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBiofilmen_US
dc.subjectDentinen_US
dc.subjectMicro-computed tomographyen_US
dc.subjectTransmission electron microscopyen_US
dc.titleVisualization and characterization ofEnterococcus faecalisbiofilm structure in bovine dentin using 2D and 3D microscopic techniquesen_US
dc.typeArticleen_US

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