Asprosin improved neuronal survival by suppressing apoptosis and enhancing the activity of the autophagy pathway in the MCAO model in rats

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dc.authorscopusid55329785600
dc.authorscopusid57221083581
dc.authorscopusid55775592400
dc.authorscopusid57193444916
dc.authorscopusid6602447584
dc.contributor.authorTanbek K.
dc.contributor.authorYuksel F.
dc.contributor.authorTekin S.
dc.contributor.authorTekin C.
dc.contributor.authorSandal S.
dc.date.accessioned2024-08-04T20:00:48Z
dc.date.available2024-08-04T20:00:48Z
dc.date.issued2024
dc.departmentİnönü Üniversitesien_US
dc.description.abstractOBJECTIVE: Cerebral ischemia (CI) is a condition in which metabolic stress increases when blood flow is interrupted in a part of the brain, resulting in oxygen and glucose deprivation. It is known that asprosin (Asp), secreted from adipose tissue during fasting, has an effect on some metabolic processes such as apoptosis, autophagy, and glucose metabolism. This study aimed to explain which of the cell death/survival Asp induces in the CI/reperfusion model. MATERIALS AND METHODS: In the study, 48 male Wistar Albino rats were divided into 6 groups: Sham, CI, Asp+CI, CI+Asp, CI+Asp+3-MA, and Asp+CI+3-MA (n=48). CI was created using the intraluminal filament technique for 60 minutes, autophagy inhibitor 3-MA (15 mg/kg/ day) and Asp (1 µg/kg/day) injections were administered 3 days before or 3 days during reperfusion. Beclin-1, ATG5, ATG7, p62, Bcl-2, Bax, active-caspase-3, and active-caspase-9 protein levels from brain tissues were determined by the Western-Blot method. The infarct area was determined by triphenyl tetrazolium chloride (TTC) staining. The Kruskal-Wallis’ test was used to compare differences between groups. p<0.05 was considered statistically significant. RESULTS: Compared to the Sham group, the increase in ischemic area and the decrease in Beclin-1, ATG-5, ATG-7, Bcl-2, Bax, active-caspase-3 and active-caspase-9 levels in the CI groups are statistically significant (p<0.05). The increase of Beclin-1, ATG-7, Bcl-2, and Bax levels in the Asp groups is statistically significant compared to the CI group (p<0.05). When Asp+CI groups and CI+Asp groups are compared, an increase in Beclin-1 levels in the Asp+CI group and the increase in Bcl-2, Bax, active-caspase-3/9 and ATG-5 levels in the CI+Asp groups are statistically significant (p<0.05). CONCLUSIONS: Asp has protective and therapeutic effects against CI/R damage. While applying Asp before ischemia activates the autophagy pathway more, applying it after ischemia protects the neuronal death/survival balance by activating the apoptosis pathway more. © 2024 Verduci Editore s.r.l. All rights reserved.en_US
dc.description.sponsorshipInönü Üniversitesi: TSG-2021-2726en_US
dc.description.sponsorshipThis research was funded by “The Research Support Unıt of Inonu University”, Project No.: TSG-2021-2726.en_US
dc.identifier.doi10.26355/eurrev_202403_35608
dc.identifier.endpage1946en_US
dc.identifier.issn1128-3602
dc.identifier.issue5en_US
dc.identifier.pmid38497877en_US
dc.identifier.scopus2-s2.0-85188045399en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1937en_US
dc.identifier.urihttps://doi.org/10.26355/eurrev_202403_35608
dc.identifier.urihttps://hdl.handle.net/11616/91022
dc.identifier.volume28en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherVerduci Editore s.r.len_US
dc.relation.ispartofEuropean Review for Medical and Pharmacological Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject3-MAen_US
dc.subjectApoptosisen_US
dc.subjectAsprosinen_US
dc.subjectAutophagyen_US
dc.subjectCerebral ischemiaen_US
dc.subjectMCAOen_US
dc.titleAsprosin improved neuronal survival by suppressing apoptosis and enhancing the activity of the autophagy pathway in the MCAO model in ratsen_US
dc.typeArticleen_US

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